Soluble peptide-MHC monomers cause activation of CD8+T cells through transfer of the peptide to T cell MHC molecules
Soluble peptide-MHC monomers cause activation of CD8+T cells through transfer of the peptide to T cell MHC molecules
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DOI：
10.1073/pnas.212515299
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发表时间：
2002-10-15
影响因子：
11.1
通讯作者：
Stern, LJ
中科院分区：
文献类型：
--
作者：
Ge, Q;Stone, JD;Stern, LJ
已知T细胞受体(TCR)介导的CD4(+)T细胞的激活需要TCR通过例如寡聚肽-MHC复合物的多价接合。相比之下，对于 CD8(+) T 细胞，有证据表明 TCR 介导的激活是通过 TCR 的单价参与来介导的。我们在这里比较了寡聚和单体 L-d 和 K-b 肽-MHC 复合物和游离肽作为表达 2C TCR 的 CD8(+) T 细胞的刺激剂。我们发现单体确实能有效激活幼稚和效应CD8(+) T细胞，但通过一种意想不到的机制，涉及肽从可溶性单体转移到T细胞内源性MHC (K-b)分子。结果是，作为抗原呈递细胞的 T 细胞能够激活其他初始 T 细胞。
T cell receptor (TCR)-mediated activation of CD4(+) T cells is known to require multivalent engagement of the TCR by, for example, oligomeric peptide-MHC complexes. In contrast, for CD8(+) T cells, there is evidence for TCR-mediated activation by univalent engagement of the TCR. We have here compared oligomeric and monomeric L-d and K-b peptide-MHC complexes and free peptide as stimulators of CD8(+) T cells expressing the 2C TCR. We found that the monomers are indeed effective in activating naive and effector CD8(+) T cells, but through an unexpected mechanism that involves transfer of peptide from soluble monomers to T cell endogenous MHC (K-b) molecules. The result is that T cells, acting as antigen-presenting cells, are able to activate other naive T cells.