Meiotic behavior of a supernumerary chromosome in Magnaporthe oryzae
Meiotic behavior of a supernumerary chromosome in Magnaporthe oryzae
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DOI：
10.1007/s00294-003-0390-7
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发表时间：
2003-06-01
期刊：
影响因子：
2.5
通讯作者：
Mayama, S
中科院分区：
文献类型：
--
作者：
Chuma, I;Tosa, Y;Mayama, S
在脉冲场凝胶中检测到一条1.2Mb的DNA带。在有丝分裂中期观察到与该带相对应的染色体实体。这种携带许多转座因子和两个端粒的微型染色体以非孟德尔方式传递到子囊孢子F，其大小和数量经常变化。RFLP标记的分离分析表明，小染色体不仅在减数分裂过程中，而且在减数分裂后都发生了结构重排，如缺失和重复。异位姐妹染色单体重组可能引起微小染色体的大小变化。
A 1.2-Mb DNA band from an isolate of Magnaporthe oryzae was detected in a pulsed-field gel. A chromosomal entity corresponding to this band was observed at the mitotic metaphase stage. This minichromosome, carrying many transposable elements and two telomeres, was transmitted to ascosporic F, cultures in a non-Mendelian manner with frequent changes in its size and number. Segregation analysis with RFLP markers indicated that the minichromosome underwent structural rearrangements, such as deletion and duplication, not only during meiosis but also after meiosis. An ectopic sister chromatid recombination may cause the size variation of the minichromosomes.