Dexamethasone reduces serum level of IL-17 in Bleomycin-A5-induced rats model of pulmonary fibrosis
Dexamethasone reduces serum level of IL-17 in Bleomycin-A5-induced rats model of pulmonary fibrosis
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DOI：
10.1080/21691401.2017.1339051
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发表时间：
2018-01-01
影响因子：
5.8
通讯作者：
Chen, Ping
中科院分区：
文献类型：
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作者：
Wang, Aihua;Wang, Fengqiang;Chen, Ping
背景：肺纤维化是一种治疗选择有限的慢性进行性疾病，炎症细胞因子在肺纤维化的发病机制中发挥着重要作用。 材料与方法：本研究中，我们研究了博来霉素-A5诱导的肺纤维化大鼠模型血清中TGF-β1、IL-8和IL-17的变化。 120只健康雄性Wistar大鼠随机分为三组：对照组（n=30）、模型组（n=45）和地塞米松（DEX）组（n=45）。模型组和DEX组大鼠经气管造口注射博莱霉素A5（5 mg/kg）诱导肺纤维化，对照组大鼠注射等量生理盐水。 DEX组大鼠术后腹腔注射DEX（4 mg/kg），每日1次。对照组和模型组大鼠均给予等量生理盐水。结果:术后第1、14、28天测定肺组织病理变化以及血清IL-8、TGF-β1、IL-17水平。博来霉素-A5 治疗后，血清 TGF-1(,) IL-8 和 IL-17 浓度显着增加，尤其是在第 14 天 (p < .01)。模型组与DEX组血清TGF-β1、IL-8水平无显着差异，但DEX治疗显着降低血清IL-17水平（p
Background: Pulmonary fibrosis is a chronic progressive disease with limited therapeutic options and inflammatory cytokines play important roles in the pathogenesis of pulmonary fibrosis.Material and method: Here, we investigated the changes of TGF-beta 1, IL-8, and IL-17 in the serum of bleomycin-A5-induced rats model of pulmonary fibrosis. 120 healthy male Wistar rats were randomly divided into three groups, the control group (n=30), the model group (n=45) and the dexamethasone (DEX) group (n=45). The rats of both model group and DEX group were injected with Bleomycin-A5 (5 mg/kg) through tracheofistulization to induce pulmonary fibrosis, while the rats of the control group were injected with equivalent physiological saline. After operation, DEX (4 mg/kg) was given to the DEX group rats intraperitoneally once a day. Equivalent saline was administered to rats of both the control group and the model group.Results: On the 1st, 14th, and 28th day after operation, pathological changes of the lung tissues, and the levels of serum IL-8, TGF-beta 1, and IL-17 were measured. The concentrations of serum TGF-1(,) IL-8, and IL-17 were significantly increased after bleomycin-A5 treatment, especially on the 14th day (p < .01). There was no significant difference between model group and DEX group in the serum level of TGF-beta 1 and IL-8, but DEX treatment significantly reduce serum IL-17 level (p