Shortwave infrared spatial frequency domain imaging for non-invasive measurement of tissue and blood optical properties.
Shortwave infrared spatial frequency domain imaging for non-invasive measurement of tissue and blood optical properties.
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DOI：
10.1117/1.jbo.27.6.066003
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发表时间：
2022-06
影响因子：
3.5
通讯作者：
Roblyer, Darren
中科院分区：
文献类型：
--
作者：
Pilvar, Anahita;Plutzky, Jorge;Pierce, Mark C.;Roblyer, Darren
短波红外 (SWIR) 光学窗口（∼900 至 2000 nm）由于光散射较低而引起了深层组织成像的兴趣。 SWIR 空间频域成像 (SWIR SFDI) 在此波长带内提供宽视场组织光学特性测量。 SWIR SFDI 的关键设计和性能特征（例如便携性、波长选择、测量分辨率和趋肤效应）尚未得到解决。制造并表征供临床使用的 SWIR SFDI 系统。根据光学特性不确定性估计和成像深度确定了波长的最佳选择。制造了一种基于紧凑型发光二极管的双波长 SWIR SFDI 系统。开发了两层逆模型来解释皮肤的分层结构。使用组织模拟模型和三名健康受试者的体内测量来验证性能。 SWIR SFDI 系统的分辨率至少为 880 nm 和 1100 nm。在模型研究中，两层逆模型将更深层提取的误差减少了至少 24%。两层模型还增加了浅表血管和周围组织之间的对比度，以进行体内测量。临床就绪的 SWIR SFDI 设备对漫射介质中微小的光学特性变化敏感，提高了体模深层光学特性量化的准确性，并增强了皮下血管的对比度。
The shortwave infrared (SWIR) optical window (∼900 to 2000 nm) has attracted interest for deep tissue imaging due to the lower scattering of light. SWIR spatial frequency domain imaging (SWIR SFDI) provides wide-field tissue optical property measurements in this wavelength band. Key design and performance characteristics, such as portability, wavelength selection, measurement resolution, and the effect of skin have not yet been addressed for SWIR SFDI. To fabricate and characterize a SWIR SFDI system for clinical use. The optimal choice of wavelengths was identified based on optical property uncertainty estimates and imaging depth. A compact light-emitting diode-based dual wavelength SWIR SFDI system was fabricated. A two-layer inverse model was developed to account for the layered structure of skin. Performance was validated using tissue-simulating phantoms and in-vivo measurements from three healthy subjects. The SWIR SFDI system had a resolution of at least at 880 nm and at 1100 nm. The two-layer inverse model reduced the error in deeper layer extractions by at least 24% in the phantom study. The two-layer model also increased the contrast between superficial vessels and the surrounding tissue for in-vivo measurements. The clinic-ready SWIR SFDI device is sensitive to small optical property alterations in diffuse media, provides enhanced accuracy in quantifying optical properties in the deeper layers in phantoms, and provided enhanced contrast of subcutaneous blood vessels.