Identification of novel metabolites in the degradation of phenanthrene by Sphingomonas sp. strain P2.
Identification of novel metabolites in the degradation of phenanthrene by Sphingomonas sp. strain P2.
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DOI：
10.1111/j.1574-6968.2000.tb09327.x
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发表时间：
2000-10
影响因子：
2.1
通讯作者：
O. Pinyakong;O. Pinyakong;H. Habe;N. Supaka;Pairoh Pinpanichkarn;K. Juntongjin;T. Yoshida;Kazuo Furihata;H. Nojiri;H. Yamane;T. Omori
中科院分区：
文献类型：
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作者：
O. Pinyakong;O. Pinyakong;H. Habe;N. Supaka;Pairoh Pinpanichkarn;K. Juntongjin;T. Yoshida;Kazuo Furihata;H. Nojiri;H. Yamane;T. Omori
从泰国石油污染土壤中分离到一株能够利用菲作为唯一碳源和能源的鞘氨醇单胞菌P2。气相色谱-质谱联用仪和核磁共振氢谱（1H）和碳谱（13 C）分析表明，菲降解途径中存在两种新的代谢产物。其中一个被鉴定为5，6-苯并香豆素，它是通过在菲的1-和2-位上进行双氧化而得到的，另一个被鉴定为1，5-二羟基-2-萘甲酸。菲降解的其它代谢产物为7，8-苯并香豆素、1-羟基-2-萘甲酸和香豆素。从这些结果可以看出，菌株P2可以通过在1，2-和3，4-位上的双氧化，然后进行间位裂解来降解菲。
Sphingomonas sp. strain P2, which is capable of utilizing phenanthrene as a sole carbon and energy source, was isolated from petroleum-contaminated soil in Thailand. Gas chromatography-mass spectrometry and (1)H and (13)C nuclear magnetic resonance analyses revealed two novel metabolites from the phenanthrene degradation pathway. One was identified as 5,6-benzocoumarin, which was derived by dioxygenation at the 1- and 2-positions of phenanthrene, and the other was determined to be 1,5-dihydroxy-2-naphthoic acid. Other metabolites from phenanthrene degradation were identified as 7, 8-benzocoumarin, 1-hydroxy-2-naphthoic acid and coumarin. From these results, it is suggested that strain P2 can degrade phenanthrene via dioxygenation at both 1,2- and 3,4-positions followed by meta-cleavage.