Effect of complex polyphenols and tannins from red wine on DNA oxidative damage of rat colon mucosa in vivo
Effect of complex polyphenols and tannins from red wine on DNA oxidative damage of rat colon mucosa in vivo
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DOI：
10.1007/s003940070013
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发表时间：
2000-10-01
影响因子：
5
通讯作者：
Dolara, P
中科院分区：
文献类型：
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作者：
Giovannelli, L;Testa, G;Dolara, P
工作背景：膳食多酚具有多种生物学作用，包括抗癌、抗氧化和抗炎活性。本研究的目的：在本研究中，我们评价了口服红酒和茶中多酚和单宁复合物对大鼠结肠粘膜DNA氧化损伤的影响。方法：从经口灌胃给予葡萄酒复合多酚（57.2mg/kg/d）或茶必精（40 mg/kg/d）处理10天的大鼠的结肠粘膜制备分离的结肠细胞。在单细胞水平上使用彗星试验technique.Results的修改分析了Colonocyte氧化DNA损伤：结果表明，葡萄酒复合多酚和单宁诱导显着降低（-62%的嘧啶和-57%的嘌呤氧化）在结肠粘膜细胞的基础DNA氧化损伤，而不影响单链断裂的基础水平。另一方面，茶多酚，即茶红素的粗提取物，不影响链断裂或嘧啶氧化在结肠mucosal cells.Conclusions我们的实验是第一次证明，膳食多酚可以调节体内氧化损伤在啮齿动物的胃肠道。这些数据支持这样的假设，即膳食多酚在氧化损伤相关的病理学中可能具有保护和治疗潜力。
Background: Dietary polyphenols have been reported to have a variety of biological actions, including anti-carcinogenic, antioxidant and anti-inflammatory activities.Aim of the study: In the present study we have evaluated the effect of an oral treatment with complex polyphenols and tannins from red wine and tea on DNA oxidative damage in the rat colon mucosa.Methods: Isolated colonocytes were prepared from the colon mucosa of rats treated for ten days with either wine complex polyphenols (57.2 mg/kg/d) or theartbigin (40 mg/kg/d) by oral gavage. Colonocyte oxidative DNA damage was analysed at the single cell level using a modification of the comet assay technique.Results: The results show that wine complex polyphenols and tannins induce a significant decrease (-62 % for pyrimidine and -57 % for purine oxidation) in basal DNA oxidative damage in colon mucosal cells without affecting the basal level of single-strand breaks. On the other hand, tea polyphenols, namely a crude extract of thearubigin, did not affect either strand breaks or pyrimidine oxidation in colon mucosal cells.Conclusions Our experiments are the first demonstration that dietary polyphenols can modulate in vivo oxidative damage in the gastrointestinal tract of rodents. These data support the hypothesis that dietary polyphenols might have both a protective and a therapeutic potential in oxidative damage-related pathologies.