miR-21 and miR-222 inhibit apoptosis of adult dorsal root ganglion neurons by repressing TIMP3 following sciatic nerve injury
miR-21 and miR-222 inhibit apoptosis of adult dorsal root ganglion neurons by repressing TIMP3 following sciatic nerve injury
复制标题
坐骨神经损伤后 miR-21 和 miR-222 通过抑制 TIMP3 抑制成人背根神经节神经元凋亡
DOI：
10.1016/j.neulet.2014.12.006
复制
发表时间：
2015-01-23
影响因子：
2.5
通讯作者：
Ding, Fei
中科院分区：
文献类型：
--
作者：
Zhou, Songlin;Zhang, Shibo;Ding, Fei
microRNAs（miRNAs或miRs）参与周围神经损伤后神经细胞的表型调节。然而，miRNAs对背根神经节（DRG）神经元存活的影响尚未得到很好的理解。在这项研究中，微阵列分析表明，在坐骨神经切断后的最初7 d期间，13种miRNA在大鼠DRG（L4-L 6）中差异表达，并且miR-21和miR-222（13种miRNA中的2种）的表达随时间持续增加。金属蛋白酶组织抑制剂3（TIMP 3）是多种癌细胞中的一种促凋亡蛋白，被确定为miR-21和miR-222的共同靶点。miR-21和miR-222的过表达抑制了培养的DRG神经元中的细胞凋亡并增强了细胞活力。IL-6可诱导miR-21表达上调。以上结果表明，miR-21和miR-222至少部分通过抑制TIMP 3抑制周围神经损伤后神经元凋亡。(C)2014爱思唯尔爱尔兰有限公司版权所有。
MicroRNAs (miRNAs or miRs) are involved in phenotype modulation of neural cells after peripheral nerve injury. The effects of miRNAs on the survival of dorsal root ganglion (DRG) neurons, however, have not yet been well understood. In this study, microarray profiling indicated that 13 miRNAs were differentially expressed in rat DRGs (L4-L6) during the initial 7 d period post sciatic nerve transection, and that the expressions of miR-21 and miR-222 (2 out of the 13 miRNAs) were continually increased over the time period. Tissue inhibitor of metalloproteinase 3 (TIMP3), a pro-apoptotic protein in various cancer cells, was identified as a common target of miR-21 and miR-222. Over-expression of miR-21 and miR-222 inhibited cell apoptosis and enhanced cell viability in cultured DRG neurons. IL-6 could induce up-regulation of miR-21 expression. All the results showed that miR-21 and miR-222 inhibited neuronal apoptosis at least partially through suppressing TIMP3 after peripheral nerve injury. (C) 2014 Elsevier Ireland Ltd. All rights reserved.