The reconstructed epidermis with melanocytes: a new tool to study pigmentation and photoprotection.
The reconstructed epidermis with melanocytes: a new tool to study pigmentation and photoprotection.
复制标题
用黑素细胞重建表皮：研究色素沉着和光保护的新工具。
DOI：
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发表时间：
1999
影响因子：
1.6
通讯作者：
A. Taïeb
中科院分区：
文献类型：
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作者：
M. Cario;S. Bessou;E. Gontier;V. Maresca;M. Picardo;A. Taïeb
在过去的十年中，结合以前的细胞培养技术，已经实现了离体表皮黑色素单元的重建。所审查的系统基本上是 Pruniéras 模型的修改，使用空气-液体界面来生长分化的角质形成细胞，在接种悬浮液中添加 5% 的黑素细胞，以及使用更适应的培养基。重复的 UVB 照射会在宏观上诱导黑色素生成的刺激，并增加重建中的黑色素浓度和黑素体转移。这些结果已用各种光型的皮肤再现。该模型可以研究表皮黑色素单元的生理学以及病理状况，如白癜风和痣。通过使用嵌合重建以及比较使用和不使用低光型高加索黑素细胞进行的自体重建，提供了角质形成细胞和黑素细胞在光保护中复杂相互作用的最新证据。基于这些发现，我们提出了对照型概念的新颖解释。
The reconstruction of the epidermal melanin unit ex vivo has been achieved during the last decade, using the combination of previous cell culture techniques. The system reviewed is basically a modification of the Pruniéras model, using the air-liquid interface to grow differentiated keratinocytes, with the addition of 5% melanocytes in the seeding suspension, as well as the use of a more adapted culture medium. Repeated UVB irradiation induces a stimulation of melanogenesis macroscopically, and increases melanin concentration and melanosome transfer in reconstructs. These results have been reproduced with skin of various phototypes. This model allows to study the physiology of the epidermal melanin unit as well as pathologic conditions, like vitiligo and nevi. Recent evidence of a complex interaction of keratinocytes and melanocytes in photoprotection was provided by the use of chimeric reconstructs and by comparing autologous reconstructs made with and without low phototype caucasoid melanocytes. Based on these findings, we suggest a novel interpretation of the concept of phototype.