Alveolar macrophage phagocytosis is impaired in children with poorly controlled asthma
Alveolar macrophage phagocytosis is impaired in children with poorly controlled asthma
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DOI：
10.1016/j.jaci.2008.03.008
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发表时间：
2008-06-01
影响因子：
14.2
通讯作者：
Brown, Lou Ann S.
中科院分区：
文献类型：
--
作者：
Fitzpatrick, Anne M.;Holguin, Fernando;Brown, Lou Ann S.
背景：下呼吸道感染是哮喘控制不良患儿的鉴别特征。目的：考虑到肺泡巨噬细胞（AMs）在先天免疫中的作用，我们假设AM吞噬功能可能在控制不良的哮喘中受损。方法：从28例哮喘患儿（中度哮喘患儿12例，重度哮喘患儿16例）、10例吸入性糖皮质激素治疗的非哮喘慢性咳嗽患儿和10例健康成人对照中分离支气管肺泡灌洗液AMs。用LPS刺激AMs，并将其暴露于异硫氰酸荧光素结合的金黄色葡萄球菌中2小时。通过吞噬细胞百分比乘以每个细胞金黄色葡萄球菌的相对荧光（RFU）单位计算的吞噬指数（PI）来量化吞噬作用。细胞凋亡是由细胞的百分比阳性的聚二磷酸腺苷核糖聚合酶测定。结果：控制不良的哮喘患者(健康对照组，9330 3992 RFU；慢性咳嗽，9042 +/- 5976 RFU；中度哮喘，4361 +/- 2536 RFU；重度哮喘，3153 +/- 1886 RFU
Background: Lower respiratory tract infection is a differentiating feature of children with poorly controlled asthma.Objective: Given the role of alveolar macrophages (AMs) in innate immunity, we hypothesized that AM phagocytosis might be impaired in poorly controlled asthma.Methods: Bronchoalveolar lavage fluid AMs were isolated from 28 asthmatic children (moderate asthma, n = 12; severe asthma, n = 16), 10 nonasthmatic children with chronic cough treated with inhaled corticosteroids, and 10 healthy adult control subjects. AMs were stimulated with LPS and exposed to fluorescein isothiocyanate-conjugated Staphylococcus aureus for 2 hours. Phagocytosis was quantified by using a phagocytic index (PI) calculated from the percentage of phagocytic cells multiplied by the relative fluorescence (RFU) units of S aureus per cell. Apoptosis was determined from the percentage of cells positive for poly (adenosine diphosphate-ribose) polymerase.Results: Phagocytosis as measured by using the unstimulated PI was decreased in subjects with poorly controlled asthma (healthy control subjects, 9330 3992 RFU; chronic cough, 9042 +/- 5976 RFU; moderate asthma, 4361 +/- 2536 RFU; severe asthma, 3153 +/- 1886 RFU; P