Characterization of the seven-day course of pulmonary response following unilateral lung acid injury in rats.
Characterization of the seven-day course of pulmonary response following unilateral lung acid injury in rats.
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DOI：
10.1371/journal.pone.0198440
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Schreiber T
中科院分区：
文献类型：
--
作者：
Setzer F;Schmidt B;Hueter L;Schwarzkopf K;Sänger J;Schreiber T
胃酸吸入是急性肺损伤的重要原因。最初48小时之后，肺部对这种损伤的反应的时间过程并不完全。本研究的目的是全面描述在直接损伤和非直接损伤的肺组织中，局灶性肺酸损伤在7天内对肺的影响。雄性Wistar大鼠进行左支气管内滴注盐酸，并在4，24，48，96或168小时后处死的侮辱。健康未受伤的动物作为对照。我们评估了左右肺灌洗液和血液中的炎性细胞计数和细胞因子水平、动脉氧分压、肺组织学改变、肺湿干重比和差异肺灌注。肺酸滴注在直接受影响的肺中诱导早期强烈的炎症反应，在4-24小时达到峰值，7天后仅部分消退。在对侧肺中观察到不太严重的反应，4天后完全消退。除IL-6外，肺泡细胞因子水平仅部分反映了肺损伤的定位以及功能和组织学改变的时间过程。肺泡白细胞亚群在酸损伤肺中表现出不同的时间进程，肺泡淋巴细胞和巨噬细胞持续升高。酸滴注后，动脉氧分压出现早期一过性下降，肺灌注优先分布于未损伤的肺。这些发现为肺酸损伤领域的进一步研究和探索机械通气对损伤肺的影响的研究提供了基础。在酸滴注后7天，直接损伤的肺不完全恢复，表明此时仍然存在对进一步伤害性刺激的脆弱性和易感性增加。
Aspiration of gastric acid is an important cause of acute lung injury. The time course of the pulmonary response to such an insult beyond the initial 48 hours is incompletely characterized. The purpose of this study was to comprehensively describe the pulmonary effects of focal lung acid injury over a seven day period in both directly injured and not directly injured lung tissue. Male Wistar rats underwent left-endobronchial instillation with hydrochloric acid and were sacrificed at 4, 24, 48, 96 or 168 h after the insult. Healthy non-injured animals served as controls. We assessed inflammatory cell counts and cytokine levels in right and left lung lavage fluid and blood, arterial oxygen tension, alterations in lung histology, lung wet-to-dry weight ratio and differential lung perfusion. Lung acid instillation induced an early strong inflammatory response in the directly affected lung, peaking at 4–24 hours, with only partial resolution after 7 days. A less severe response with complete resolution after 4 days was seen in the opposite lung. Alveolar cytokine levels, with exception of IL-6, only partially reflected the localization of lung injury and the time course of the functional and histologic alterations. Alveolar leucocyte subpopulations exhibited different time courses in the acid injured lung with persistent elevation of alveolar lymphocytes and macrophages. After acid instillation there was an early transient decrease in arterial oxygen tension and lung perfusion was preferentially distributed to the non-injured lung. These findings provide a basis for further research in the field of lung acid injury and for studies exploring effects of mechanical ventilation on injured lungs. Incomplete recovery in the directly injured lung 7 days after acid instillation suggests that increased vulnerability and susceptibility to further noxious stimuli are still present at that time.
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影响因子：
19.7
作者：
Goodman, LR;Fumagalli, R;Pesenti, A
通讯作者：
Pesenti, A
影响因子：
8.3
作者：
Chang, Chia-Yu;Cheng, Tain-Junn;Kawachi, Ichiro
通讯作者：
Kawachi, Ichiro
影响因子：
8.8
作者：
GATTINONI, L;PESENTI, A;TORRESIN, A
通讯作者：
TORRESIN, A
影响因子：
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作者：
GOLDMAN, G;WELBOURN, R;HECHTMAN, HB
通讯作者：
HECHTMAN, HB
影响因子：
8.8
作者：
KNIGHT, PR;DRUSKOVICH, G;JOHNSON, KJ
通讯作者：
JOHNSON, KJ