Sequence analysis of an Australian isolate of sugarcane bacilliform badnavirus
Sequence analysis of an Australian isolate of sugarcane bacilliform badnavirus
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DOI：
10.1007/s00705-002-0879-2
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发表时间：
2002-12-01
影响因子：
2.7
通讯作者：
Smith, GR
中科院分区：
文献类型：
--
作者：
Geijskes, RJ;Braithwaite, KS;Smith, GR
对甘蔗杆状病毒（SCBV IM）澳大利亚分离物的基因组进行了克隆、测序和分析。基因组由7687个核苷酸组成，含有3个开放的阅读框，其大小和组织与其他Badnaviruses相似。SCBV-IM与SCBV-Morocco分离物在ORF 1、11和III编码区的氨基酸序列相似性分别为91.4%、83.8%和85.3%。SCBV IM ORF III推导的氨基酸序列的系统发育分析表明，SCBV分离物之间的关系比其他Badnaviruses更密切。从三个不同的甘蔗品种的SCBV序列的扩增显示相当大的变异性的病毒种群，无论是在单一感染的植物以及感染的植物之间，这表明SCBV分离物测序的日期可能不代表的病毒变异的范围。
The genome of an Australian isolate of Sugarcane bacilliform virus (SCBV-IM) was cloned, sequenced and analysed. The genome consisted of 7687 nucleotides and contained three open reading frames which were similar in size and organisation to those of other badnaviruses. SCBV-IM was found to be most similar to the SCBV-Morocco isolate with amino acid sequence similarity of 91.4%, 83.8% and 85.3% in the ORF 1, 11 and III coding regions, respectively. Phylogenetic analysis of the SCBV-IM ORF III deduced amino acid sequence showed that SCBV isolates were more closely related to each other than to other badnaviruses. Amplification of SCBV sequences from three different sugarcane varieties revealed considerable variability in the viral populations, both within single infected plants as well as between infected plants, suggesting that the SCBV isolates sequenced to date may not be representative of the range of virus variability.