Cografts of adrenal medulla with C6 glioma cells in rats with 6-hydroxydopamine-induced lesions.
Cografts of adrenal medulla with C6 glioma cells in rats with 6-hydroxydopamine-induced lesions.
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具有 6-羟基多巴胺诱导损伤的大鼠的肾上腺髓质与 C6 神经胶质瘤细胞的共同移植物。
DOI：
10.1016/0306-4522(90)90175-4
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发表时间：
1990
期刊：
影响因子：
3.3
通讯作者：
Gash,DM
中科院分区：
文献类型：
--
作者：
Bing,G;Notter,MF;Hansen,JT;Kellogg,C;Kordower,JH;Gash,DM
为了提高嗜铬细胞的存活率和表型分化，将已知能产生神经营养因子(S)的无丝分裂[~3H]胸腺嘧啶核苷标记的C6胶质瘤细胞单独移植到肾上腺嗜铬细胞，并与肾上腺嗜铬细胞共同移植。用6-羟基多巴胺造成黑质纹状体通路损伤的Long-Evans大鼠分为4组：(1)肾上腺髓质细胞与C6胶质瘤细胞共移植组；(2)单独接受肾上腺髓质移植组；(3)单独接受C6胶质瘤移植组；(4)作为载体对照组。移植后1个月处死所有大鼠。采用免疫组织化学、神经化学和放射自显影等方法鉴定和表征移植细胞。在所有单独接受肾上腺髓质移植或肾上腺髓质与C6胶质瘤细胞联合移植的动物中，都发现了酪氨酸羟基酶免疫反应细胞。与仅接受肾上腺嗜铬细胞移植的宿主相比，联合移植受者有更多的酪氨酸羟化酶免疫反应细胞(P<；0.05)。此外，在前一组中，更多的移植嗜铬细胞形成突起。所有三个组织受体组(肾上腺髓质、C6胶质瘤细胞和联合移植动物)在苯丙胺(0.5 mg/kg ip)后同侧旋转显著减少(P<；0.05)。与对照组相比，注射。此外，共移植宿主的旋转减少比其他两个移植组更明显(P<；0.05)。在共移植和肾上腺髓质移植受者的移植部位发现显著高于假移植对照动物的多巴胺水平。
Amitotic [3H]thymidine-labeled C6 glioma cells, which are known to produce neurotrophic factor(s), were grafted alone and with adrenal chromaffin cells in an attempt to improve chromaffin cell survival and phenotypic differentiation. Long-Evans rats with unilateral 6-hydroxydopamine-induced lesions of the nigrostriatal pathway were divided into four groups: (1) those receiving adrenal medullary cells co-transplanted with C6 glioma cells; (2) those receiving adrenal medullary graft alone; (3) those receiving C6 glioma grafts alone; and (4) those serving as a vehicle control group. All rats were killed one month after transplantation. Immunohistochemical, neurochemical, and autoradiographic methods were used to identify and characterize the grafted cells. Tyrosine hydroxylase-immunoreactive cells were found in all animals that received grafts of the adrenal medulla alone or of adrenal medulla co-transplanted with C6 glioma cells. The cograft recipients had more tyrosine hydroxylase-immunoreactive cells than the hosts receiving just adrenal chromaffin cells (P< 0.05). Additionally, more grafted chromaffin cells formed processes in the former group. All three tissue recipient groups (adrenal medullary, C6 glioma cell, and cografted animals) had a significant reduction (P< 0.05) in ipsilateral rotations after amphetamine (0.5 mg/kg i.p.) injections as compared to the control vehicle recipient group. Moreover, the reduction in rotation was more marked in the cografted hosts than in the other two implanted groups (P< 0.05).Significantly higher dopamine levels were found in the transplant sites of both cograft and adrenal medullary graft recipients than in sham grafted control animals.