DNA damage profiles induced by sunlight at different latitudes
DNA damage profiles induced by sunlight at different latitudes
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DOI：
10.1002/em.21678
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发表时间：
2012-04-01
影响因子：
2.8
通讯作者：
Martins Menck, Carlos Frederico
中科院分区：
文献类型：
--
作者：
Schuch, Andre Passaglia;Yagura, Teiti;Martins Menck, Carlos Frederico
尽管人们越来越了解紫外线辐射对人类健康和生态系统的生物影响，但在全球变暖和气候变化的情况下，仍然难以预测太阳紫外线辐射发生率增加的负面影响。因此，开发和应用基于DNA的生物传感器来监测不同环境条件下的太阳紫外辐射具有越来越重要的意义。为了从分子角度研究阳光的遗传毒性影响，在南美洲不同纬度地区用DNA剂量计系统进行了实地实验，同时进行了太阳UVB/UVA辐射的物理光度测定。在应用基于特异性DNA修复酶和抗体的生物化学和免疫学方法来评估阳光诱导的DNA损伤概况时，很明显，阳光的遗传毒性潜力确实因纬度而异。尽管如此，虽然氧化的DNA碱基的诱导直接依赖于纬度的增加，但6- 4PP的产生相反，因此后者可以被认为是UVB发病率的生物分子标志物。这种分子DNA损伤模式在很大程度上反映了UVA和UVB能量在任何特定纬度的相对发生率。由此证明了这种基于DNA的生物传感器的适用性，额外的，连续的现场实验，作为一种手段，登记的变化，在太阳紫外线辐射的遗传毒性的影响。Environ.摩尔变异体2012. (c)2012 Wiley Periodicals，Inc.
Despite growing knowledge on the biological effects of ultraviolet (UV) radiation on human health and ecosystems, it is still difficult to predict the negative impacts of the increasing incidence of solar UV radiation in a scenario of global warming and climate changes. Hence, the development and application of DNA-based biological sensors to monitor the solar UV radiation under different environmental conditions is of increasing importance. With a mind to rendering a molecular view-point of the genotoxic impact of sunlight, field experiments were undertaken with a DNA-dosimeter system in parallel with physical photometry of solar UVB/UVA radiation, at various latitudes in South America. Onapplying biochemical and immunological approaches based on specific DNA-repair enzymes and antibodies, for evaluating sunlight-induced DNA damage profiles, it became clear that the genotoxic potential of sunlight does indeed vary according to latitude. Notwithstanding, while induction of oxidized DNA bases is directly dependent on an increase in latitude, the generation of 6-4PPs is inversely so, whereby the latter can be regarded as a biomolecular marker of UVB incidence. This molecular DNA lesion-pattern largely reflects the relative incidence of UVA and UVB energy at any specific latitude. Hereby is demonstrated the applicability of this DNA-based biosensor for additional, continuous field experiments, as a means of registering variations in the genotoxic impact of solar UV radiation. Environ. Mol. Mutagen. 2012. (c) 2012 Wiley Periodicals, Inc.