Cell surface recycling of internalized antigen permits dendritic cell priming of B cells
Cell surface recycling of internalized antigen permits dendritic cell priming of B cells
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DOI：
10.1016/j.immuni.2005.09.013
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发表时间：
2005-11-01
期刊：
影响因子：
32.4
通讯作者：
Clyne, R
中科院分区：
文献类型：
--
作者：
Bergtold, A;Desai, DD;Clyne, R
树突状细胞处理内化的抗原，将降解产物呈现在MHC上，以识别TCR。由于抗原暴露的DC也可诱导体液免疫，因此DC还必须保留其天然状态的抗原以使BCR与B细胞结合。在这里，我们证明了由抑制性Fc受体Fc-Gamma RIIB内吞的抗原进入一个非降解的细胞内囊泡室，该囊泡室循环到细胞表面，使天然抗原与B细胞上的BCR相互作用。用免疫球蛋白调理的T非依赖性抗原免疫，可增强FcyRIIB和补体依赖的体液免疫反应。运送到脾边缘带的IC负载DC可以Fc-γ-RIIB依赖的方式启动T非依赖性反应。因此，树突状细胞配备了非降解和降解的抗原摄取途径，以促进抗原呈递给B细胞和T细胞。
Dendritic cells process internalized antigens to present degradative products on MHC for TCR recognition. Because antigen-exposed DCs also induce humoral immunity, DCs must also retain antigen in its native state for the engagement of BCR on B cells. Here, we demonstrate that antigen endocytosed by the inhibitory Fc receptor, Fc gamma RIIB, accesses a nondegradative intracellular vesicular compartment that recycles to the cell surface, enabling interaction of native antigen with BCR on B cells. Immunization with IgG-opsonized, T independent antigens leads to enhanced humoral responses in a FcyRIIB and complement dependent manner. IC-loaded DCs trafficking to the splenic marginal zone can prime a T independent response in an Fc gamma RIIB-dependent manner. Thus dendritic cells are equipped with both nondegradative and degradative antigen uptake pathways to facilitate antigen presentation to both B and T cells.