High-purity capture of CTCs based on micro-beads enhanced isolation by size of epithelial tumor cells (ISET) method
High-purity capture of CTCs based on micro-beads enhanced isolation by size of epithelial tumor cells (ISET) method
复制标题
基于微珠的上皮肿瘤细胞大小增强分离 (ISET) 方法高纯度捕获 CTC
DOI：
10.1016/j.bios.2017.11.026
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发表时间：
2018-04-15
影响因子：
12.6
通讯作者：
Pei, Renjun
中科院分区：
文献类型：
--
作者：
Sun, Na;Li, Xinpan;Pei, Renjun
在本文中，我们开发了一种基于尺寸的低成本微流控芯片，使用传统的聚碳酸酯膜从血液中分离CTC，并提出了一种通过利用与CTC特异性结合的修饰微珠的尺寸放大方法来提高细胞过滤前捕获效率的策略。使用我们的微流体捕获系统以 1 mL/min 的流速从全血样本中分离出高达 91% 的靶细胞。此外，还引入了白细胞去除过程，大大减少了滤膜上保留的白细胞。对膜上捕获的细胞进行免疫荧光分析测试，表明该装置可以提供可靠的全血样本中的 CTC 识别和 CTC 计数。最后，该设备在从癌症患者血液样本中检测 CTC 方面得到了进一步验证，这表明检测 CTC 对治疗反应的能力很有前景。
In this paper, we develop a low-cost size-based microfluidic chip using conventional polycarbonate membrane to isolate CTCs from blood, and propose a strategy to increase the capture efficiency before cell filtration by a size enlargement method utilizing modified microbeads specifically binding to CTCs. Up to 91% of target cells were isolated from whole blood samples using our microfluidic capture system at a flow rate of 1 mL/min. Moreover, a WBC depletion process is introduced which greatly decreases the WBC retaining on the filter membrane. The tests of immunofluorescence analysis of cells captured on the membrane were performed, which demonstrates that the device could provide a dependable CTC identification and CTC count in whole blood samples. Finally, the device was further validated in the detection of CTCs from blood samples of cancer patients, and it indicates a promising capability to detect CTC response to treatment.