Regulation of HPV16 E6 and MCL1 by SF3B1 inhibitor in head and neck cancer cells.
Regulation of HPV16 E6 and MCL1 by SF3B1 inhibitor in head and neck cancer cells.
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DOI：
10.1038/srep06098
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发表时间：
2014-08-20
影响因子：
4.6
通讯作者：
Koide K
中科院分区：
文献类型：
--
作者：
Gao Y;Trivedi S;Ferris RL;Koide K
ABT-737 inhibits the anti-apoptotic proteins B-cell lymphoma 2 (BCL-2) and BCL-XL. Meayamycin B switches the splicing pattern of myeloid cell leukemia factor 1 (MCL1) pre-mRNA. Specifically, inhibition of splicing factor 3B subunit 1 (SF3B1) with meayamycin B promotes the generation of the proapoptotic, short splicing variant (MCL1-S) and diminishes the antiapoptotic, long variant (MCL1-L). This action was previously associated with the cytotoxicity of meayamycin B in non-small cell lung carcinoma cell lines. ABT-737 induced apoptosis in response to an ablation of MCL1-L by meayamycin B. In this study, we further exploited this synergistic combination in head and neck squamous cell carcinoma (HNSCC), up to 90% of which overexpress MCL1 and BCL-XL. In a panel of seven HNSCC cell lines, the combination of meayamycin B and ABT-737 rapidly triggered a Bax/Bak-mediated apoptosis that overcame the resistance from HPV16-positive HNSCC against each agent alone. Both RT-PCR and Western blotting showed that meayamycin B up-regulated MCL1-S and down-regulated MCL1-L. Significantly, we discovered that SF3B1 was involved in the splicing of oncogenic HPV16 E6 to produce non-oncogenic HPV16 E6*, indicating that SF3B1 may inhibit HPV16-induced tumorigenesis.
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影响因子：
8.4
作者：
Ferris, RL;Martinez, I;Khan, S
通讯作者：
Khan, S
DOI：
10.1002/hed.10274
发表时间：
2003-08-01
影响因子：
2.9
作者：
Bradford, CR;Zhu, SB;Carey, TE
通讯作者：
Carey, TE
DOI：
10.1093/jnci/djk179
发表时间：
2007-05-16
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
Hashibe, Mia;Brennan, Paul;Boffetta, Paolo
通讯作者：
Boffetta, Paolo
影响因子：
9
作者：
Laetsch TW;Liu X;Vu A;Sliozberg M;Vido M;Elci OU;Goldsmith KC;Hogarty MD
通讯作者：
Hogarty MD
影响因子：
158.5
作者：
KOUTSKY, LA;HOLMES, KK;KIVIAT, NB
通讯作者：
KIVIAT, NB