Assessing the toxicity of contaminated soils using the nematode Caenorhabditis elegans as test organism
Assessing the toxicity of contaminated soils using the nematode Caenorhabditis elegans as test organism
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DOI：
10.1016/j.ecoenv.2009.07.003
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发表时间：
2009-10-01
影响因子：
6.8
通讯作者：
Roembke, J.
中科院分区：
文献类型：
--
作者：
Hoess, S.;Jaensch, S.;Roembke, J.
本研究以秀丽隐杆线虫为测试生物，对9种未污染的参考土壤和22种具有不同理化性质和污染模式的污染土壤进行了标准化毒性测试。土壤中的线虫的肥力，生长和繁殖进行了比较，在标准土壤Lufa St.2.2。线虫表现出100%的肥力和一个非常低的变异性增长的参考土壤。尽管不同参照土壤的繁殖情况差异很大，但所有参照土壤均符合有效性标准（每种试验生物> 30个后代）。此外，Lufa St. 2.2被证明是一种合适的和具有代表性的对照土壤。为了明确分类污染土壤对线虫的影响，毒性阈值推导出线虫生育力（20%抑制），生长（10%抑制）和繁殖（40%抑制）的基础上测试固有的变异性（MDD =最小可检测的差异），以及它们之间的变异性未污染的参考土壤（MTI =最大可容忍的抑制）。污染土壤对线虫有明显的毒害作用，但其毒性与土壤有机质的相关性比与重金属污染的相关性好。有趣的是，线虫毒性试验的结果与在相同土壤中进行的寡毛类、弹尾虫和植物试验的结果没有很好的相关性，这表明结果并不多余。用线虫进行的毒性试验结果表明，它适用于野外采集土壤的毒性试验，并可能成为土壤试验电池的一个有价值的补充。(C)2009 Elsevier Inc. All rights reserved.
In this study, nine uncontaminated reference soils and 22 contaminated soils with different physicochemical properties and contamination patterns were tested with a standardized toxicity test, using the nematode, Caenorhabditis elegans, as test organism. Fertility, growth and reproduction of C elegans in the soils were compared with the exposure in standard soil Lufa St.2.2. C elegans showed 100% fertility and a very low variability of growth in the reference soils. Although, reproduction varied considerably between the various reference soils, validity criteria (> 30 offspring per test organism) were met in all reference soils. Moreover, Lufa St. 2.2 turned out to be a suitable and representative control soil. In order to clearly classify the effects of the polluted soils on C elegans, toxicity thresholds were derived for nematode fertility (20% inhibition), growth (10% inhibition) and reproduction (40% inhibition) on the basis of the test inherent variability (MDD = minimal detectable difference), as well as their variability between the uncontaminated reference soils (MTI = maximal tolerable inhibition). The contaminated soils showed clear toxic effects on the nematodes, whereas the toxicity was better correlated to organic than to heavy metal contamination in bulk soil. Interestingly, the results of the nematode toxicity test were not well correlated with those of tests with oligochaetes, collembolans and plants, performed with the same soils, showing that the results are not redundant. The toxicity test using C elegans turned out to be suitable for testing the toxicity of field collected soils and might by a valuable addition to soil test batteries. (C) 2009 Elsevier Inc. All rights reserved.