Division plane placement in pleomorphic archaea is dynamically coupled to cell shape.
Division plane placement in pleomorphic archaea is dynamically coupled to cell shape.
复制标题
多形性古细菌中的划分平面位置与细胞形状动态耦合。
DOI：
10.1111/mmi.14316
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发表时间：
2019
影响因子：
3.6
通讯作者：
Curmi,PaulMG
中科院分区：
文献类型：
--
作者：
Walsh,JamesC;Angstmann,ChristopherN;Bisson-Filho,AlexandreW;Garner,EthanC;Duggin,IainG;Curmi,PaulMG
实现细胞分裂机器的精确定位的一种机制是通过图灵模式，其中非线性分子相互作用自发地产生时空浓度梯度。由此产生的图案由细胞形状决定。例如，大肠杆菌的Min系统显示细胞两极之间的时空振荡，留下一个中间细胞区进行分裂。目前尚不清楚分裂放置模式形成机制的普遍性。我们研究了两个多形性古菌，Haloferax volcanii和Haloarcula japonica的分裂平面的位置，并表明它与图灵模式的预测相关。H. volcanii表明，连续几轮分裂后的分裂位置是由子细胞形状动态决定的。为H。volcanii，我们表明，DNA的位置不影响分裂平面的位置，排除了类核闭塞。三角形细胞提供了一个严格的测试图灵图案，其中有一个分歧，在分裂平面的方向。对于两个古生菌检查，大多数三角形细胞分裂预测的图灵机制，然而，在某些情况下，观察到多个分裂平面导致细胞分裂成三个可行的后代。我们的研究结果表明，该司网站的位置是一致的图灵图案系统在这些古菌。
One mechanism for achieving accurate placement of the cell division machinery is via Turing patterns, where nonlinear molecular interactions spontaneously produce spatiotemporal concentration gradients. The resulting patterns are dictated by cell shape. For example, the Min system ofEscherichia colishows spatiotemporal oscillation between cell poles, leaving a mid‐cell zone for division. The universality of pattern‐forming mechanisms in divisome placement is currently unclear. We examined the location of the division plane in two pleomorphic archaea,Haloferax volcaniiandHaloarcula japonica, and showed that it correlates with the predictions of Turing patterning. Time‐lapse analysis ofH. volcaniishows that divisome locations after successive rounds of division are dynamically determined by daughter cell shape. ForH. volcanii, we show that the location of DNA does not influence division plane location, ruling out nucleoid occlusion. Triangular cells provide a stringent test for Turing patterning, where there is a bifurcation in division plane orientation. For the two archaea examined, most triangular cells divide as predicted by a Turing mechanism; however, in some cases multiple division planes are observed resulting in cells dividing into three viable progeny. Our results suggest that the division site placement is consistent with a Turing patterning system in these archaea.