Dual-modality optical coherence tomography and frequency-domain fluorescence lifetime imaging microscope system for intravascular imaging.
Dual-modality optical coherence tomography and frequency-domain fluorescence lifetime imaging microscope system for intravascular imaging.
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DOI：
10.1117/1.jbo.25.9.096010
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发表时间：
2020-09
影响因子：
3.5
通讯作者：
Applegate B
中科院分区：
文献类型：
--
作者：
Chen X;Kim W;Serafino M;Tan Z;Jo J;Applegate B
重要性：详细的生化和形态学成像的斑块负荷的冠状动脉持有的承诺，提高认识的动脉粥样硬化斑块的发展，最终导致更好的诊断和治疗。目的：开发双模态血管内导管，支持UV激发内源性荧光团的扫频源光学相干断层扫描（OCT）和频域荧光寿命成像（FD-FLIM）。方法：我们制定了一个完善的方法，内窥镜的发展，结合模拟在一个商业射线跟踪程序，制造，并优化球透镜性能的测量方法。通过这种方法，我们设计并开发了一种基于双包层光纤的双模态导管内窥镜，该双包层光纤通过纤芯支持OCT，通过第一包层支持荧光收集。我们改变了发射到纤芯和第一包层的UV激发的相对百分比，以探索FD-FLIM的潜在分辨率改进。所开发的导管内窥镜的光学特性，包括空间分辨率和标准荧光团的荧光寿命的测量。最后，该系统被证明在新鲜的离体人体冠状动脉。结果：所开发的内窥镜被证明具有与从模拟方法获得的预测相似的光学性能。FLIM分辨率可以通过主要通过纤芯而不是第一包层照射而提高超过4倍。然而，在选择相对百分比时，需要考虑与时间相关的日晒损失。我们最终选择用7%的能量通过核心传输。所得导管内窥镜具有OCT的横向分辨率和FD-FLIM的横向分辨率。离体冠状动脉的图像与基于组织病理学的预期一致。结论：结果表明，我们的内窥镜模拟方法可以对内窥镜性能进行可靠的预测。模拟结果指导了我们开发多模态OCT/FD-FLIM导管成像系统，用于研究冠状动脉粥样硬化。
Significance: Detailed biochemical and morphological imaging of the plaque burdened coronary arteries holds the promise of improved understanding of atherosclerosis plaque development, ultimately leading to better diagnostics and therapies. Aim: Development of a dual-modality intravascular catheter supporting swept-source optical coherence tomography (OCT) and frequency-domain fluorescence lifetime imaging (FD-FLIM) of endogenous fluorophores with UV excitation. Approach: We instituted a refined approach to endoscope development that combines simulation in a commercial ray tracing program, fabrication, and a measurement method for optimizing ball-lens performance. With this approach, we designed and developed a dual-modality catheter endoscope based on a double-clad fiber supporting OCT through the core and fluorescence collection through the first cladding. We varied the relative percent of UV excitation launched into the core and first cladding to explore the potential resolution improvement for FD-FLIM. The developed catheter endoscope was optically characterized, including measurement of spatial resolution and fluorescent lifetimes of standard fluorophores. Finally, the system was demonstrated on fresh ex vivo human coronary arteries. Results: The developed endoscope was shown to have optical performance similar to predictions derived from the simulation approach. The FLIM resolution can be improved by over a factor of 4 by primarily illuminating through the core rather than the first cladding. However, time-dependent solarization losses need to be considered when choosing the relative percentage. We ultimately chose to illuminate with 7% of the power transmitting through the core. The resulting catheter endoscope had lateral resolution for OCT and lateral resolution for FD-FLIM. Images of ex vivo coronary arteries are consistent with expectations based on histopathology. Conclusions: The results demonstrate that our approach for endoscope simulation produces reliable predictions of endoscope performance. Simulation results guided our development of a multimodal OCT/FD-FLIM catheter imaging system for investigating atherosclerosis in coronary arteries.