Tpx-1 is a component of the outer dense fibers and acrosome of rat spermatozoa
Tpx-1 is a component of the outer dense fibers and acrosome of rat spermatozoa
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DOI：
10.1002/1098-2795(200101)58:1
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发表时间：
2001-01-01
影响因子：
2.5
通讯作者：
De Kretser, DM
中科院分区：
文献类型：
--
作者：
O'Bryan, MK;Sebire, K;De Kretser, DM
以前，我们报道了一个成员的富含半胱氨酸的分泌蛋白家族，tpx-1，从睾丸表达文库使用外致密纤维（ODF）特异性抗血清的克隆。使用免疫组织化学和免疫电镜技术和蛋白质印迹纯化的精子尾部组件，我们已经确定，tpx-1存在作为25和27 kDa的蛋白在两个组成部分的大鼠精子细胞：ODF和顶体。Tpx-1 mRNA首先在粗线期晚期精母细胞中表达，但这些tpx-1蛋白的产生被延迟4-5天，然后被掺入发育中的精子顶体中，包围伸长和浓缩的精子细胞核。在精子头部形成的同时，tpx-1蛋白被掺入发育中的精子尾部，特别是ODF。tpx-1蛋白被认为是在结构内类似于粒状体在细胞质叶的精子细胞延长，并随后纳入不断增长的尾巴，以符合ODF发展的方式。此外，tpx-1蛋白定位于超微结构水平的连接件的颈部和纵列的纤维鞘，这表明在这些细胞骨架结构的共同蛋白质成分。因此，tpx-1可能在精子头部发育和尾部功能的过程中具有功能意义。(C)2001 Wiley-Liss，Inc.
Previously we reported the cloning of a member of the cysteine-rich secretory protein family, tpx-1, from a testis expression library using an outer dense fiber (ODF)-specific antiserum. Using immunohistochemical and immunoelectron microscopic techniques and Western blotting of purified sperm tail components, we have determined that tpx-1 exists as 25 and 27 kDa proteins in two components of rat spermatid: the ODFs and the acrosome. Tpx-1 mRNA is first expressed in the late pachytene spermatocytes, but the production of these tpx-1 proteins is translationally delayed for 4-5 days before being incorporated into the developing sperm acrosome, surrounding the elongating and condensing spermatid nucleus. Concurrent with sperm head formation, tpx-1 protein was incorporated into the developing sperm tail, and specifically the ODFs. The tpx-1 protein was seen within structures resembling granulated bodies in the cytoplasmic lobe of elongating spermatids and was incorporated subsequently into the growing tail in a manner consistent with ODF development. In addition, tpx-1 protein was localized at the ultrastructural level of the connecting piece of the neck and longitudinal columns of the fibrous sheath, suggesting common protein components in these cytoskeletal structures. As such, tpx-1 may have functional significance in the processes of sperm head development and tail function. (C) 2001 Wiley-Liss, Inc.