Broad-spectrum detection of papillomaviruses in bovine teat papillomas and healthy teat skin
Broad-spectrum detection of papillomaviruses in bovine teat papillomas and healthy teat skin
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DOI：
10.1099/vir.0.80086-0
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发表时间：
2004-08-01
影响因子：
3.8
通讯作者：
Shirasawa, H
中科院分区：
文献类型：
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作者：
Ogawa, T;Tomita, Y;Shirasawa, H
为了研究牛乳头瘤病毒（BPV）在牛乳头瘤和健康皮肤中的流行情况，使用引物对FAP 59/FAP 64和MY 09/MY 11通过PCR分析从乳头瘤和健康乳头皮肤拭子提取的DNA。使用FAP 59/FAP 64在所有15份乳头状瘤标本中检测到乳头状瘤病毒（PV）DNA，使用MY 09/MY 11在8/15份乳头状瘤标本中检测到PV DNA。在拭子样本中，使用FAP 59/FAP 64和MY 09/MY 11分别检测122份样本中的21份和8份为PV DNA阳性。在39份病毒DNA阳性标本中发现了4种BPV型（BPV-1、-3、-5和-6）、2种已知的BPV型（BAA 1和-5）和11种新的PV型（命名为BAPV 1 ~-10和BAPV 11 MY）。将推定的新PV类型与已报道的BPV进行氨基酸序列比对，并将推定的新PV类型与人和动物PV类型进行系统发育分析，结果表明BAPV 1至-10和BAPV 11 MY是推定的新BPV类型。这些结果也显示了BPV基因组的多样性和亚临床感染的程度。
To investigate the prevalence of bovine papillomavirus (BPV) in bovine papilloma and healthy skin, DNA extracted from teat papillomas and healthy teat skin swabs was analysed by PCR using the primer pairs FAP59/FAP64 and MY09/MY11. Papillomavirus (PV) DNA was detected in all 15 papilloma specimens using FAP59/FAP64 and in 8 of the 15 papilloma specimens using MY09/MY11. In swab samples, 21 and 8 of the 122 samples were PV DNA positive using FAP59/FAP64 and MY09/MY11, respectively. Four BPV types (BPV-1, -3, -5 and -6), two previously identified putative BPV types (BAA1 and -5) and 11 putative new PV types (designated BAPV1 to -10 and BAPV1 1 MY) were found in the 39 PV DNA-positive samples. Amino acid sequence alignments of the putative new PV types with reported BPVs and phylogenetic analyses of the putative new PV types with human and animal PV types showed that BAPV1 to -10 and BAPV1 1 MY are putative new BPV types. These results also showed the genomic diversity and extent of subclinical infection of BPV.