Analysis of gene function in the zebrafish retina
Analysis of gene function in the zebrafish retina
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DOI：
10.1016/s1046-2023(02)00262-1
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发表时间：
2002-12-01
期刊：
影响因子：
4.8
通讯作者：
Pujic, Z
中科院分区：
文献类型：
--
作者：
Malicki, J;Jo, HY;Pujic, Z
斑马鱼的诱变筛选发现了数百个影响视网膜发育的突变等位基因，并将斑马鱼确立为脊椎动物眼睛发育的主要模型之一。除了正向遗传诱变方法外，还使用几种反向遗传技术研究斑马鱼胚胎中的基因功能。其中一些依赖于基因产物的过度表达，另一些则利用反义寡核苷酸来阻断选定基因座的功能。在这里，我们在眼睛发育的背景下描述这些方法。 (C) 2002 年爱思唯尔科学（美国）。版权所有。
Mutagenesis screens in zebrafish have uncovered several hundred mutant alleles affecting the development of the retina and established the zebrafish as one of the leading models of vertebrate eye development. In addition to forward genetic mutagenesis approaches, gene function in the zebrafish embryo is being studied using several reverse genetic techniques. Some of these rely on the overexpression of a gene product, others take advantage of antisense oligonucleotides to block function of selected loci. Here we describe these methods in the context of the developing eye. (C) 2002 Elsevier Science (USA). All rights reserved.