Hesperidin Effects on Gut Microbiota and Gut-Associated Lymphoid Tissue in Healthy Rats
Hesperidin Effects on Gut Microbiota and Gut-Associated Lymphoid Tissue in Healthy Rats
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DOI：
10.3390/nu11020324
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发表时间：
2019-02-01
期刊：
影响因子：
5.9
通讯作者：
Camps-Bossacoma, Mariona
中科院分区：
文献类型：
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作者：
Estruel-Amades, Sheila;Massot-Cladera, Malen;Camps-Bossacoma, Mariona
柑橘类水果中发现的橙皮苷具有广泛的生物学特性。尽管如此，对免疫系统的影响，特别是对肠道相关淋巴组织的影响，以及它与肠道微生物群的关系，还需要进行更深入的研究。因此，我们旨在确定口服橙皮苷对健康动物肠道淋巴组织和肠道微生物群组成的影响。大鼠口服橙皮苷100或200 mg/kg，每周3次，连续4周。测定盲肠内容物的菌群组成和iga包被菌群。评估肠系膜淋巴结淋巴细胞（MLNL）组成和功能。定量测定小肠中IgA、细胞因子和基因表达。橙皮苷处理导致细菌和iga包被细菌数量增加，微生物群组成发生变化，如乳酸杆菌比例增加。橙皮苷还能提高小肠IgA含量。小肠的这些变化伴随着干扰素和单核细胞趋化蛋白-1浓度的降低。此外，橙皮苷增加了MLNL中TCR+淋巴细胞的相对比例。这些结果表明橙皮苷对肠道相关淋巴组织的免疫调节作用，并加强了其作为益生元的作用。
Hesperidin, found in citrus fruits, has shown a wide range of biological properties. Nonetheless, a more in-depth investigation is required on the effects on the immune system, and in particular, on the gut-associated lymphoid tissue, together with its relationship with the gut microbiota. Therefore, we aimed to establish the influence of oral hesperidin administration on the intestinal lymphoid tissue and on the gut microbiota composition in healthy animals. Lewis rats were orally administrated 100 or 200 mg/kg hesperidin three times per week for four weeks. Microbiota composition and IgA-coated bacteria were determined in caecal content. Mesenteric lymph node lymphocyte (MLNL) composition and functionality were assessed. IgA, cytokines, and gene expression in the small intestine were quantified. Hesperidin administration resulted in a higher number of bacteria and IgA-coated bacteria, with changes in microbiota composition such as higher Lactobacillus proportion. Hesperidin was also able to increase the small intestine IgA content. These changes in the small intestine were accompanied by a decrease in interferon- and monocyte chemotactic protein-1 concentration. In addition, hesperidin increased the relative proportion of TCR+ lymphocytes in MLNL. These results show the immunomodulatory actions of hesperidin on the gut-associated lymphoid tissue and reinforce its role as a prebiotic.