REGULATION AND SELECTIVE EXPRESSION OF LY-6A/E, A LYMPHOCYTE-ACTIVATION MOLECULE, IN THE CENTRAL-NERVOUS-SYSTEM
REGULATION AND SELECTIVE EXPRESSION OF LY-6A/E, A LYMPHOCYTE-ACTIVATION MOLECULE, IN THE CENTRAL-NERVOUS-SYSTEM
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DOI：
10.1016/0169-328x(90)90003-v
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发表时间：
1990-06-01
期刊：
影响因子：
--
通讯作者：
LEVY, RB
中科院分区：
文献类型：
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作者：
CRAY, C;KEANE, RW;LEVY, RB
Ly-6基因座编码一组主要在小鼠淋巴细胞上发现的细胞表面分子。这些蛋白质与Thy-1（一种在淋巴组织和神经组织中表达的分子）具有几种结构和功能特征。利用抗Ly-6A/E单克隆抗体，本研究结果表明这些分子在脑组织中的原位表达。研究结果还表明，这些分子在胚胎或新生儿发育阶段不表达。此外，尽管Ly-6 b单倍型小鼠表现出主要与整个脑中的血管成分相关的染色，但Ly-6a小鼠表现出主要与限于海马和中脑区域的白色物质相关的染色。尽管培养的神经胶质细胞和神经元细胞表达最低可检测水平的Ly-6A/E，但这些培养物中的大多数GFAP+细胞在与包括rIFN-γ在内的细胞因子孵育后表达高水平的Ly-6A/E。此外，北方印迹分析的RNA从丰富的星形胶质细胞培养证实了诱导的Ly-6 A/E表达。因此，这些发现已经确定Ly-6是在脑和淋巴组织中表达的基因组之一。
The Ly-6 locus encodes a group of cell surface molecules found predominately on lymphoid cells in the mouse. These proteins share several structural and functional characteristics with Thy-1, a molecule expressed in both lymphoid and neuronal tissue. Utilizing anti-Ly-6A/E monoclonal antibodies, the present results demonstrate in situ expression of these molecules in brain tissue. The findings also indicated that these molecules are not expressed during embryonic or neonatal stages of development. Moreover, although Ly-6b haplotype mice exhibited staining primary associated with vascular elements throughout the brain, Ly-6a mice exhibited staining predominantly associated with white matter limited to the hippocampal and midbrain regions. Although cultured glial and neuronal cells expressed marginally detectable levels of Ly-6A/E, the majority of GFAP+ cells in these cultures expressed high levels of Ly-6A/E following incubation with cytokines including rIFN-gamma. In addition, northern blot analysis of RNA from enriched astrocytic cultures corroborated the induction of Ly-6A/E expression. These findings have therefore established that Ly-6 is amongst those groups of genes expressed in both brain and lymphoid tissues.