INCREASED EXPRESSION OF A 58-KDA PROTEIN-KINASE LEADS TO CHANGES IN THE CHO CELL-CYCLE
INCREASED EXPRESSION OF A 58-KDA PROTEIN-KINASE LEADS TO CHANGES IN THE CHO CELL-CYCLE
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DOI：
10.1073/pnas.87.19.7467
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发表时间：
1990-10-01
影响因子：
11.1
通讯作者：
KIDD, VJ
中科院分区：
文献类型：
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作者：
BUNNELL, BA;HEATH, LS;KIDD, VJ
我们已经分离和表征了编码人58-kDa蛋白激酶的cDNA，该蛋白激酶与细胞分裂控制（CDC）蛋白激酶同源。这种蛋白激酶还含有一个独特的N-末端结构域，可能潜在地调节其功能。由于其与p34 CDC 2的相关性，在CHO细胞中过表达人p58 cDNA以确定对细胞周期的影响。p58在这些细胞中的高表达导致细胞周期的晚期末期和早期G1期延长。这些p58过表达者表现出微管蛋白中间体的频率显著增加以及有丝分裂异常的显著增加。因此，p58蛋白激酶的适当调节对于这些细胞中的正常细胞周期进展是必不可少的。
We have isolated and characterized cDNA encoding a human 58-kDa protein kinase that is homologous to the cell division control (CDC) protein kinases. This protein kinase also contains a unique N-terminal domain that may potentially regulate its function. Due to its relatedness to p34CDC2, the human p58 cDNA was overexpressed in CHO cells to determine the effect on the cell cycle. Elevated expression of p58 in these cells resulted in prolonged late telophase and early G1 phase of the cell cycle. These p58 overexpressors showed a significantly increased frequency of tubulin midbodies as well as significant increases in mitotic abnormalities. Thus, proper regulation of p58 protein kinase is essential for normal cell cycle progression in these cells.