Plasma separation PMMA device driven by capillary force controlling surface wettability
Plasma separation PMMA device driven by capillary force controlling surface wettability
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DOI：
10.1049/mnl.2011.0627
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发表时间：
2012-01
影响因子：
1.3
通讯作者：
H. Sakamoto;R. Hatsuda;K. Miyamura;S. Sugiyama
中科院分区：
文献类型：
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作者：
H. Sakamoto;R. Hatsuda;K. Miyamura;S. Sugiyama
在这封信中，毛细作用驱动的微器件开发的高通量芯片上分离血浆从一滴血。微流体装置由2米深和2米宽的聚甲基丙烯酸甲酯通道阵列组成。它是由热压和热粘结从硅主模具。然后，使用氧等离子体和聚-L-赖氨酸对其进行改性以获得亲水性表面。在注入血液样品后，血浆快速流动并在3分钟内到达出口，证明红细胞分离比大于99。此外，未观察到溶血。由于所提出的装置仅由毛细作用驱动，因此其被动操作模式不需要微流体泵或任何其他额外的电源。因此，它可以被用于诊断系统中。
In this Letter, a microdevice driven by capillary action is developed for the high-throughput on-chip separation of plasma from a drop of blood. The microfluidic device consists of an array of polymethyl methacrylate channels 2 m deep and 2 m wide. It is fabricated by hot embossing and thermal bonding from a Si master mold. Then, it is modified using oxygen plasma and poly-l-lysine to obtain a hydrophilic surface. After the injection of a blood sample, the plasma flows rapidly and arrives at the outlet within 3 min, demonstrating a red blood cell separation ratio greater than 99 . Moreover, no haemolysis was observed. Since the proposed device is driven solely by capillary action, its passive mode of operation requires no microfluidic pumps or any other additional power supply. It can therefore be miniaturised for incorporation in a diagnostic system.