Distribution of Biocide Resistance Genes and Association with Clonal Complex Genotypes in Staphylococcus aureus Isolated from School-Age Children in Guangzhou.
Distribution of Biocide Resistance Genes and Association with Clonal Complex Genotypes in Staphylococcus aureus Isolated from School-Age Children in Guangzhou.
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DOI：
10.2147/idr.s387528
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发表时间：
2022
影响因子：
3.9
通讯作者：
中科院分区：
文献类型：
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作者：
在受影响的社区，经常给儿童开氯己定和莫匹罗星，以防止金黄色葡萄球菌的定植和传播，但这导致了杀菌剂耐药性的上升。本研究旨在了解广州地区学龄儿童金黄色葡萄球菌对杀菌剂的耐药性基因分布，研究携带杀菌剂耐药性基因的菌株对葡萄糖酸氯己定和莫匹罗星的敏感性和克隆复合物（CC）基因型，并进一步探讨生物膜在这种耐药性中的作用。对已有的722株金黄色葡萄球菌进行耐药和多位点序列基因分型。采用PCR法测定9个杀菌剂基因（qacA/B、mupA、mepA、sepA、norA、lmrS、smr、mupB、qacG）的分布。进一步检测携带qacA/B或mupA基因的分离株对葡萄糖酸氯己定（CHG）和莫匹罗星的敏感性和生物膜形成能力。9个耐药基因中以mepA（95.57%）最多，其次为norA（78.81%）、lmrS（77.01%）和sepA（58.17%）。未检测到qacG基因。sepA在CC30和CC45基因型中的分布明显减少，sepA的存在与对CLI、ERY、TCY、SXT、CIP和LVX等抗生素的耐药有关。此外，64株（94.1%,n=68） qacA/B+菌株对CHG耐药，12株（100.0%,n=12） mupA+菌株对莫匹罗星耐药，4株（80%,n=5）和5株（100%,n=5） qacA/B+菌株对CHG和莫匹罗星耐药。85株分离菌中，98.8%（84株）具有不同程度的生物膜形成能力，与CHG和莫匹罗星耐药性呈正相关。抗杀菌剂基因的分布与特定的cc有关。qacA/B和mupA基因与CHG和莫匹罗星的抗性高度相关，生物膜的形成有助于这种杀菌剂抗性。
Chlorhexidine and mupirocin are often prescribed to children in affected communities to prevent colonization and transmission of Staphylococcus aureus, but this has led to an increasing rate of biocide resistance. In this study, we aimed to determine the distribution of biocide resistance genes among S. aureus isolates from school-age children in Guangzhou, investigate chlorhexidine gluconate and mupirocin susceptibility and clonal complex (CC) genotypes in strains carrying biocide-resistance genes, and further explore the role of biofilms in this resistance. Antibiotic resistance and multilocus sequence genotyping were performed on 722 S. aureus isolates from previous study. The distribution of nine biocide genes (qacA/B, mupA, mepA, sepA, norA, lmrS, smr, mupB, qacG) was determined by PCR. Isolates carrying qacA/B or mupA genes were further tested for susceptibility to chlorhexidine gluconate (CHG) and mupirocin and biofilm formation abilities. The most prevalent of the nine biocide resistance genes were mepA (95.57%), followed by norA (78.81%), lmrS (77.01%), and sepA (58.17%). The qacG gene was not detected. Distribution of sepA was significantly decreased in CC30 and CC45 genotypes, and presence of sepA was associated with resistance to antibiotics such as CLI, ERY, TCY, SXT, CIP, and LVX. In addition, 64 (94.1%, n=68) qacA/B+ isolates showed CHG resistance, 12 (100.0%, n=12) mupA+ isolates were mupirocin resistant, and 4 (80%, n=5) and 5 (100%, n=5) qacA/B+mupA+ isolates were CHG and mupirocin resistant, respectively. Of these 85 isolates, 98.8% (n=84) had different degrees of biofilm-forming abilities, which were positively associated with CHG and mupirocin resistance. The distribution of biocide resistance genes was associated with special CCs. The qacA/B and mupA genes are highly associated with resistance to CHG and mupirocin, and biofilm formation was found to contribute to this biocide resistance.