Fermentation of fructooligosaccharides and inulin by bifidobacteria: a comparative study of pure and fecal cultures
Fermentation of fructooligosaccharides and inulin by bifidobacteria: a comparative study of pure and fecal cultures
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DOI：
10.1128/aem.71.10.6150-6158.2005
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发表时间：
2005-10-01
影响因子：
4.4
通讯作者：
Matteuzzi, D
中科院分区：
文献类型：
--
作者：
Rossi, M;Corradini, C;Matteuzzi, D
研究了55株双歧杆菌对低聚果糖和菊粉的利用情况。而大多数菌株都能发酵果寡糖，而以菊粉为碳源时，只有8株能生长。采用高效阴离子交换层析-脉冲安培检测法对分批发酵后的残留碳水化合物进行分析。观察到菌株对不同长度的果聚糖具有依赖的降解能力。在菊粉分批发酵过程中，短果糖先消失，长果糖逐渐消耗。然而，生长通过一个不间断的指数阶段发生，没有表现出与链长相关的多项式行为。在所有测试的21株双歧杆菌中都发现了细胞β-呋喃果糖苷酶。对4株菌株进行了胞外降解果糖活性的测定，只有发酵菊粉的2株菌株具有胞外降解果糖的活性。用人类粪便浆液接种的分批培养证实了低聚果糖和菊粉的双歧化作用，并表明其他肠道微生物群也以这些碳源为基础生长。我们观察到，双歧杆菌以菊粉肠道降解菌产生的单糖和低聚糖交叉喂养生长，粪便上清液的高水解性证明了这一点。果寡糖和菊粉对粪便培养物中短链脂肪酸的产生有较大影响，菊粉的主要发酵产物为丁酸，而醋酸盐和乳酸主要由果寡糖发酵产生。
The utilization of fructooligosaccharides (FOS) and inulin by 55 Bifidobacterium strains was investigated. Whereas FOS were fermented by most strains, only eight grew when inulin was used as the carbon source. Residual carbohydrates were analyzed by high-performance anion-exchange chromatography with pulsed amperometric detection after batch fermentation. A strain-dependent capability to degrade fructans of different lengths was observed. During batch fermentation on inulin, the short fructans disappeared first, and then the longer ones were gradually consumed. However, growth occurred through a single uninterrupted exponential phase without exhibiting polyauxic behavior in relation to the chain length. Cellular beta-fructofuranosidases were found in all of the 21 Bifidobacterium strains tested. Four strains were tested for extracellular hydrolytic activity against fructans, and only the two strains which ferment inulin showed this activity. Batch cultures inoculated with human fecal slurries confirmed the bifidogenic effect of both FOS and inulin and indicated that other intestinal microbial groups also grow on these carbon sources. We observed that bifidobacteria grew by cross-feeding on mono- and oligosaccharides produced by primary inulin intestinal degraders, as evidenced by the high hydrolytic activity of fecal supernatants. FOS and inulin greatly affected the production of short-chain fatty acids in fecal cultures; butyrate was the major fermentation product on inulin, whereas mostly acetate and lactate were produced on FOS.