Microbial Community Dynamics and Assembly Follow Trajectories of an Early-Spring Diatom Bloom in a Semienclosed Bay
Microbial Community Dynamics and Assembly Follow Trajectories of an Early-Spring Diatom Bloom in a Semienclosed Bay
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DOI：
10.1128/aem.01000-18
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发表时间：
2018-07
影响因子：
4.4
通讯作者：
Huajun Zhang;Kai Wang;Lixin Shen;Heping Chen;Fanrong Hou;Xiaoyan Zhou;Demin Zhang;Xiangyu Zhu
中科院分区：
文献类型：
--
作者：
Huajun Zhang;Kai Wang;Lixin Shen;Heping Chen;Fanrong Hou;Xiaoyan Zhou;Demin Zhang;Xiangyu Zhu
有害藻华（HAB）显着影响细菌驱动的生物地球化学循环。赤潮期间微生物群落的变化已被深入研究，但赤潮期间微生物群落的组装机制知之甚少，有限的调查确定性和随机过程的平衡，在形成赤潮中的微生物群落。本研究对早春硅藻水华过程中微生物群落的动态和聚集进行了研究。我们的数据既证实了以前观察到的一般微生物演替模式，并显示了新的详细机制，微生物组装在赤潮。这些结果将有助于更深入地了解形成赤潮微生物群落的生态过程。此外，在这项研究中的代谢潜力的预测将有助于了解在海洋环境中的赤潮氮代谢的影响。摘要赤潮是严重的生态灾害，严重影响着由细菌驱动的海洋地球化学循环。赤潮期间微生物群落的变化已被广泛研究，但赤潮期间微生物群落的组装机制知之甚少。在这里，使用16 S rRNA基因扩增子测序，我们分析了早春硅藻水华期间的微生物群落，以研究微生物组装过程的动态。在水华期间，细菌科以杜鹃花科、黄杆菌科和微杆菌科为主。30个最丰富的操作分类单位（OTU）根据特定的开花阶段分为4个集群，在开花过程中表现出明显的演替模式。微生物群落的演替与藻类物种动态的变化相关。基于β-最近分类距离构建的模拟模型表明，在水华初期，微生物群落的聚集表现为强异质选择性，在水华中期表现为随机性，在水华后期和水华后表现为强同质选择性。这些演替主要是由叶绿素a含量驱动的，而叶绿素a含量主要受中肋骨条藻的影响。微生物群落功能预测表明，微生物代谢功能与氮代谢密切相关。总之，我们的研究结果清楚地表明，在赤潮中的微生物群落组装的决定性的主导作用，并将促进更深入地了解的生态过程中形成的微生物群落在水华过程中。重要性有害藻华（HABs）显著影响由细菌驱动的生态地球化学循环。赤潮期间微生物群落的变化已被深入研究，但赤潮期间微生物群落的组装机制知之甚少，有限的调查确定性和随机过程的平衡，在形成赤潮中的微生物群落。本文研究了早春硅藻水华发生过程中微生物群落的动态和聚集。我们的数据既证实了以前观察到的一般微生物演替模式，并显示了新的详细机制，微生物组装在赤潮。这些结果将有助于更深入地了解形成赤潮微生物群落的生态过程。此外，在这项研究中的代谢潜力的预测将有助于了解在海洋环境中的赤潮氮代谢的影响。
Harmful algal blooms (HABs) significantly influence biogeochemical cycles driven by bacteria. The shifts in microbial communities during HABs have been studied intensively, but the assembly mechanisms of microbial communities during HABs are poorly understood, with limited investigation of the balance of deterministic and stochastic processes in shaping microbial communities in HABs. In this study, the dynamics and assembly of microbial communities in an early-spring diatom bloom process were investigated. Our data both confirm previously observed general microbial successional patterns and show new detailed mechanisms for microbial assembly in HABs. These results will facilitate deeper understanding of the ecological processes shaping microbial communities in HABs. In addition, predictions of metabolic potential in this study will facilitate understanding of the influence of HABs on nitrogen metabolism in marine environments. ABSTRACT Harmful algal blooms (HABs) are serious ecological disasters in coastal areas, significantly influencing biogeochemical cycles driven by bacteria. The shifts in microbial communities during HABs have been widely investigated, but the assembly mechanisms of microbial communities during HABs are poorly understood. Here, using 16S rRNA gene amplicon sequencing, we analyzed the microbial communities during an early-spring diatom bloom, in order to investigate the dynamics of microbial assembly processes. Rhodobacteraceae, Flavobacteriaceae, and Microbacteriaceae were the main bacterial families during the bloom. The 30 most abundant operational taxonomic units (OTUs) segregated into 4 clusters according to specific bloom stages, exhibiting clear successional patterns during the bloom process. The succession of microbial communities correlated with changes in the dynamics of algal species. Based on the β-nearest taxon distance, we constructed a simulation model, which demonstrated that the assembly of microbial communities shifted from strong heterogenous selection in the early stage of the bloom to stochasticity in the middle stage and then to strong homogeneous selection in the late and after-bloom stages. These successions were driven mainly by chlorophyll a contents, which were affected mainly by Skeletonema costatum. Moreover, functional prediction of microbial communities showed that microbial metabolic functions were significantly related to nitrogen metabolism. In summary, our results clearly suggested a dominant role of determinacy in microbial community assembly in HABs and will facilitate deeper understanding of the ecological processes shaping microbial communities during the algal bloom process. IMPORTANCE Harmful algal blooms (HABs) significantly influence biogeochemical cycles driven by bacteria. The shifts in microbial communities during HABs have been studied intensively, but the assembly mechanisms of microbial communities during HABs are poorly understood, with limited investigation of the balance of deterministic and stochastic processes in shaping microbial communities in HABs. In this study, the dynamics and assembly of microbial communities in an early-spring diatom bloom process were investigated. Our data both confirm previously observed general microbial successional patterns and show new detailed mechanisms for microbial assembly in HABs. These results will facilitate deeper understanding of the ecological processes shaping microbial communities in HABs. In addition, predictions of metabolic potential in this study will facilitate understanding of the influence of HABs on nitrogen metabolism in marine environments.