GGF/neuregulin is a neuronal signal that promotes the proliferation and survival and inhibits the differentiation of oligodendrocyte progenitors
GGF/neuregulin is a neuronal signal that promotes the proliferation and survival and inhibits the differentiation of oligodendrocyte progenitors
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DOI：
10.1016/s0896-6273(00)80155-5
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发表时间：
1996-08-01
期刊：
影响因子：
16.2
通讯作者：
Salzer, JL
中科院分区：
文献类型：
--
作者：
Canoll, PD;Musacchio, JM;Salzer, JL
我们发现，GGF/neuregulin是促少突胶质细胞（O 4 +/O 1-细胞），少突胶质细胞（O 4 +/O 1+细胞）和2型星形胶质细胞的有丝分裂原。调蛋白β 1，另一种神经调蛋白亚型，也是促有丝分裂的。胶质生长因子（GGF）的增殖作用不需要血清因子，但血清因子可大大增强其增殖作用。GGF还促进前少突胶质细胞在无血清条件下的存活。高水平的GGF可逆地抑制少突胶质细胞祖细胞的分化和谱系定型，并且在分化的培养物中，导致O 1和髓鞘碱性蛋白表达的丧失。所有三种erbB受体都由祖细胞表达并被GGF激活;这些受体的相对丰度在分化过程中发生变化。最后，皮质神经元释放一种可溶性的促少突胶质细胞有丝分裂原，这种促少突胶质细胞有丝分裂原被GGF抗体特异性阻断。这些结果表明neuregulins参与了少突胶质细胞祖细胞增殖、存活和分化的神经调节。
We show that GGF/neuregulin is a mitogen for pro-oligodendrocytes (O4+/O1- cells), oligodendrocytes (O4+/O1+ cells), and type-2 astrocytes. Heregulin beta 1, another neuregulin isoform, is also mitogenic. The proliferative effect of glial growth factor (GGF) does not require, but is greatly potentiated by, serum factors. GGF also promotes the survival of pro-oligodendrocytes under serum-free conditions. High levels of GGF reversibly inhibit the differentiation and lineage commitment of oligodendrocyte progenitors and, in differentiated cultures, result in loss of O1 and myelin basic protein expression. All three erbB receptors are expressed by progenitors and are activated by GGF; the relative abundance of these receptors changes during differentiation. Finally, cortical neurons release a soluble mitogen for pro-oligodendrocytes that is specifically blocked by antibodies to GGF. These results implicate the neuregulins in the neuronal regulation of oligodendrocyte progenitor proliferation, survival, and differentiation.