Determination of enrofloxacin and ciprofloxacin in foods of animal origin by capillary electrophoresis with field amplified sample stacking-sweeping technique
Determination of enrofloxacin and ciprofloxacin in foods of animal origin by capillary electrophoresis with field amplified sample stacking-sweeping technique
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DOI：
10.1016/j.foodchem.2014.12.054
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发表时间：
2015-06-01
期刊：
影响因子：
8.8
通讯作者：
Shu, Yang
中科院分区：
文献类型：
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作者：
Xu, Xiaoying;Liu, Lihong;Shu, Yang
建立了场放大样堆积(FASS)与扫描相结合的在线预富集方法，用于多种食品中恩诺沙星和环丙沙星的检测。为了提高扫描效率，在样品基质中引入了γ-环糊精(γ-CD)。优化的实验条件为：运行缓冲液(pH 8.8)，20 mM Tris和80 mM NaDC，进样时间300 S；b，20 mM Tris，120 mM NaDC，进样240次S，样品基质，300 mM Tris，5 mMγ-CD，pH 10.0，外加电压15 kV，检测波长270 nm。在缓冲条件(A)下，Enro和CIP的检出限分别为1.87和2.21 ng/m L。与传统的毛细管电泳法相比，Enro和CIP的灵敏度分别提高了376倍和406倍。条件(B)的缓冲液已成功地应用于毛细管电泳联用紫外检测器测定六种动物性食品。加标回收率为85-102%，相对标准偏差为0.1-4.4%。(C)2014爱思唯尔有限公司。保留所有权利。
A simple on-line preconcentration method combining field-amplified sample stacking (FASS) with sweeping was developed and validated for the determination of enrofloxacin (ENRO) and ciprofloxacin (CIP) in multiple foods. To improve the efficiency of sweeping, gamma-cyclodextran (gamma-CD) was introduced to the sample matrix. The optimal experimental conditions were as follows: running buffer (pH 8.8), (a) 20 mM Tris and 80 mM NaDC with 300 s sample injection time, (b) 20 mM Tris and 120 mM NaDC with 240 s sample injection time; sample matrix, 300 mM Tris and 5 mM gamma-CD at pH 10.0; applied voltage, 15 kV; detection wavelength, 270 nm. With buffer condition (a), the limits of detection (LODs) for ENRO and CIP were 1.87 and 2.21 ng/mL. The sensitivity was improved 376 and 406-fold for ENRO and CIP compared to conventional capillary electrophoresis (CE) method, respectively. The buffer with condition (b) has been successfully applied to the analysis of six kinds of animal foodstuffs by capillary electrophoresis with UV detector. The recoveries (85-102%) were achieved with relative standard deviations of 0.1-4.4%. (C) 2014 Elsevier Ltd. All rights reserved.