Highly selective and stable glucose biosensor based on incorporation of platinum nanoparticles into polyaniline-montmorillonite hybrid composites
Highly selective and stable glucose biosensor based on incorporation of platinum nanoparticles into polyaniline-montmorillonite hybrid composites
复制标题
基于将铂纳米粒子掺入聚苯胺-蒙脱土杂化复合材料的高选择性和稳定的葡萄糖生物传感器
DOI：
10.1016/j.microc.2019.104266
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发表时间：
2020
影响因子：
4.8
通讯作者：
Chen Jianfang
中科院分区：
文献类型：
--
作者：
Zheng Hao;Liu Maokun;Yan Zupeng;Chen Jianfang
生物传感器的选择性和稳定性仍然是一个具有挑战性的目标。采用原位电化学悬浮聚合法在铂电极表面制备聚苯胺（PANI）-蒙脱土（MMT）-铂纳米粒子（PtNPs）杂化复合材料，并通过电沉积法将PtNPs固定在聚苯胺-蒙脱土-铂纳米粒子纳米复合材料上，制备了葡萄糖氧化酶（GOD）生物传感器。采用扫描电子显微镜（SEM）、循环伏安法（CV）、电化学阻抗谱（EIS）和电流法对所制备的生物传感器的形貌和电化学性能进行表征。没有监测到来自可能的干扰物如甘氨酸（Gly）、尿素（Urea）、L-苯丙氨酸（L-Phe）、抗坏血酸（AA）、L-酪氨酸（L-Tyr）和D-半乳糖（D-Gal）的电流响应。葡萄糖检测信号强度在储存40天后几乎没有变化，即使在两个月后仍然保持在原始值的91.7%以上。该传感器对葡萄糖的响应速度快，线性范围从10 μM到1.94 mM，检测限为0.1 μM。该传感器已成功应用于人血清中葡萄糖的检测。结果表明，利用电化学合成方法可以控制纳米复合材料的微环境，使葡萄糖氧化酶在分子水平上分散，增强电子传递和电催化效果。
The selectivity and stability of biosensor are still challenging goal. Herein, a glucose biosensor was developed by anchoring glucose oxidase (GOD) within the polyaniline (PANI)-montmorillonite (MMT)-platinum nanoparticles (PtNPs) nanocomposite through the electrodeposition of PtNPs on the PANI-MMT hybrid composites, which had been prepared on the surface of platinum plate electrode by in situ electrochemical suspension polymerization. Scanning electron spectroscopy (SEM), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and amperometry measurements were employed to characterize the morphology and electrochemical properties of the as-prepared biosensor. No current response was monitored from the possible interferents such as glycine (Gly), urea (Urea), L-phenylalanine (L-Phe), ascorbic acid (AA), L-tyrosine (L-Tyr) and D-galactose (D-Gal). The signal intensity towards glucose detection kept almost no change after storage for 40 days, and still remained above 91.7% of original value even after two months. The developed biosensor revealed a quick response to glucose over a wide linear range from 10 μM to 1.94 mM and a low detection limit of 0.1 μM. The biosensor was successfully applied for glucose detection in human blood serum. The results demonstrate the possible control of nanocomposite microenvironment by using electrochemical synthesis to obtain molecular level dispersion for immobilizing glucose oxidase, and enhance electron transfer and electrocatalytic effect.