Micro-Computed Tomographical Imaging of Soft Biological Materials Using Contrast Techniques
Micro-Computed Tomographical Imaging of Soft Biological Materials Using Contrast Techniques
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DOI：
10.1089/ten.tec.2008.0436
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发表时间：
2009-09-01
影响因子：
3
通讯作者：
Daamen, Willeke F.
中科院分区：
文献类型：
--
作者：
Faraj, Kaeuis A.;Cuijpers, Vincent M. J. I.;Daamen, Willeke F.
这项工作的目的是介绍由软天然生物材料制成的支架的高分辨率计算机断层扫描(Micro-CT)，并将这些数据与传统的扫描电子显微镜和光学显微镜技术进行比较。以胶原基支架为例。与矿化组织不同，胶原支架不能为Micro-CT成像提供足够的X射线衰减。因此，使用两种结构不同的支架，一种是圆孔的，另一种是单向片层的，应用和评价了各种金属基造影剂。获得高分辨率三维图像的最佳对比度技术要么是四氧化三和醋酸铀酰的组合，要么是醋酸铀酰和柠檬酸铅的组合。显微CT分析得到的数据与光学显微镜和电子显微镜得到的数据相一致。然而，由于Micro-CT设备光斑尺寸的限制，无法显示小结构(小于几mm)。总之，使用适当的对比方案，可以获得由柔软的天然生物材料制备的支架的可靠的三维图像。这将微CT分析的使用扩展到软材料，如基于蛋白质的生物材料。
The aim of this work was to introduce high-resolution computed tomography (micro-CT) for scaffolds made from soft natural biomaterials, and to compare these data with the conventional techniques scanning electron microscopy and light microscopy. Collagen-based scaffolds were used as examples. Unlike mineralized tissues, collagen scaffolds do not provide enough X-ray attenuation for micro-CT imaging. Therefore, various metal-based contrast agents were applied and evaluated using two structurally distinct scaffolds, one with round pores and one with unidirectional lamellae. The optimal contrast techniques for obtaining high-resolution three-dimensional images were either a combination of osmium tetroxide and uranyl acetate, or a combination of uranyl acetate and lead citrate. The data obtained by micro-CT analysis were in line with data obtained by light and electron microscopy. However, small structures (less than a few mm) could not be visualized due to limitation of the spot size of the micro-CT apparatus. In conclusion, reliable three-dimensional images of scaffolds prepared from soft natural biomaterials can be obtained using appropriate contrast protocols. This extends the use of micro-CT analysis to soft materials, such as protein-based biomaterials.