Decreased expression of the vitamin D receptor in women with recurrent pregnancy loss
Decreased expression of the vitamin D receptor in women with recurrent pregnancy loss
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反复流产的女性体内维生素 D 受体的表达降低
DOI：
10.1016/j.abb.2016.07.021
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发表时间：
2016-09-15
影响因子：
3.9
通讯作者：
Yu, Xuewen
中科院分区：
文献类型：
--
作者：
Yan, Xiaoting;Wang, Liqin;Yu, Xuewen
维生素D-3的多种功能激发了人们对这种维生素在怀孕期间可能发挥的作用的兴趣。本研究调查了妊娠早期妇女维生素D受体(VDR)的表达，以确定VDR是否与复发性妊娠丢失(RPL)有关。40例妊娠7~10周的RPL妇女和40例胎龄相近的健康孕妇作为研究对象。采用免疫组织化学、激光共聚焦扫描显微镜、免疫印迹和实时定量聚合酶链式反应等方法检测绒毛和蜕膜中VDR基因和蛋白的表达。采用双抗体夹心酶联免疫吸附试验检测血清VDR水平。RPL患者绒毛和蜕膜组织中VDR mRNA的表达明显低于对照组(P均<0.0001)。Western印迹分析显示，与对照组相比，RPL组绒毛中VDR的表达减少了约46%，蜕膜中VDR的表达减少了52%。RPL组血清维生素D受体水平也显著低于对照组(p=0.003)。免疫组织化学和激光共聚焦显微镜检测显示，VDR在绒毛滋养细胞和基质细胞、蜕膜腺上皮细胞和基质细胞中的表达均显著低于对照组(均P<0.05)。综上所述，这些观察结果表明，RPL患者绒毛、蜕膜和血清中VDR的表达水平均低于正常妊娠妇女，提示妊娠早期VDR表达降低可能与RPL有关。(C)2016 Elsevier Inc.保留所有权利。
The multiple functions of vitamin D-3 have stimulated interest in the role that this vitamin may play during pregnancy. The present study investigated the expression of the vitamin D receptor (VDR) in women during the first trimester of pregnancy in order to determine whether VDR is associated with recurrent pregnancy loss (RPL). Forty women at 7-10 weeks gestation with RPL and 40 women of similar gestational age with a healthy pregnancy were recruited. VDR mRNA and protein in chorionic villi and decidua were evaluated by immunohistochemistry, confocal laser scanning microscopy (CLSM), western blot, and quantitative real-time polymerase chain reaction. The serum levels of VDR were measured by an enzyme-linked immunosorbent assay. Women with RPL had a significantly weaker expression of VDR mRNA in villi and decidual tissues compared with the control women (both p < 0.0001). Western blot analysis showed an approximately 46% decrease in VDR expression in villi and a 52% decrease in decidua in the RPL vs. the controls. Serum VDR levels were also significantly lower in the RPL group than in the control group (p = 0.003). Compared with the controls, immunohistochemical and CLSM analysis revealed significantly lower VDR expression in villous cytotrophoblasts and stromal cells, as well as in decidual glandular epithelial and stromal cells (all p < 0.05). In conclusion, these observations show that women with RPL have lower levels of VDR expression in chorionic villi, decidua and serum compared with normal pregnant women, suggesting that decreased VDR expression in the first trimester pregnancy may be associated with RPL. (C) 2016 Elsevier Inc. All rights reserved.