Preparative isolation and purification of alkaloids from Corydalis yanhusuo W. T.!Wang by high speed counter-current chromatography
Preparative isolation and purification of alkaloids from Corydalis yanhusuo W. T.!Wang by high speed counter-current chromatography
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DOI：
10.1080/10826070500274638
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发表时间：
2005-01-01
影响因子：
1.3
通讯作者：
Lou, JZ
中科院分区：
文献类型：
--
作者：
Tong, SQ;Yan, JZ;Lou, JZ
从延胡索总生物碱中分离得到4种生物碱：脱氢延胡索碱、巴马汀、黄连碱和Columbamine。T. Wang使用通过高速逆流色谱法（HSCCC）的逐步洗脱法。采用四氯化碳-三氯甲烷-甲醇-0.2mol/L盐酸（1：7：3：4，v/v/v/v）和三氯甲烷-甲醇-0.2mol/L盐酸（7：3：4，v/v/v）组成的两相溶剂体系，从200 mg总生物碱中一次性提取出4种相对纯的生物碱。脱氢可的松、巴马汀、黄连碱和Columbamine的纯度分别为99.1、97.1、99.7和94.9%。其结构经H-1 NMR和C-13 NMR确证。
Four alkaloids including dehydrocorydaline, palmatine, coptisine, and columbamine were successfully isolated from the total alkaloids of Corydalis yanhusuo W. T. Wang using a stepwise elution by high speed counter-current chromatography (HSCCC). A pair of two-phase solvent systems consisting of carbon tetrachloride-trichloromethane-methanol-0.2 mol/L hydrochloric acid (1 : 7 : 3 : 4, v/v/v/v) and trichloromethane-methanol-0.2 mol/L hydrochloric acid (7 : 3 : 4, v/v/v) was successively used, which yielded four relatively pure alkaloids from 200 mg of the total alkaloids in a single run. The purities of dehydrocorydaline, palmatine, coptisine, and columbamine were 99.1, 97.1, 99.7, and 94.9%, respectively. The structures were identified by H-1 NMR and C-13 NMR.