A MACROPHAGE FACTOR INHIBITS ADIPOCYTE GENE-EXPRESSION - AN INVITRO MODEL OF CACHEXIA
A MACROPHAGE FACTOR INHIBITS ADIPOCYTE GENE-EXPRESSION - AN INVITRO MODEL OF CACHEXIA
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DOI：
10.1126/science.3839597
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
RINGOLD, GM
中科院分区：
文献类型：
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作者：
TORTI, FM;DIECKMANN, B;RINGOLD, GM
哺乳动物中的某些感染和恶性肿瘤导致称为恶病质的病症的发展，其中动物持续减轻体重，通常同时消耗足够的饮食。当巨噬细胞被内毒素刺激时，它们产生一种或多种称为cachetin的因子，其抑制培养的脂肪细胞中的脂肪产生（脂肪生成）酶的活性。这种效应可能反映了恶病质的生理基础之一。在本研究中，来自在脂肪细胞分化过程中表达增加的基因的互补DNA克隆被用于研究恶病质凝集素作用的分子基础。在恶病质存在下，相应基因的表达被可逆地和特异性地抑制。当成熟的脂肪细胞暴露于恶病质时，这些基因的mRNA减少并迅速接近分化前的水平。
Certain infections and malignancies in mammals cause the development of a condition known as cachexia in which the animal continues to lose weight, often while consuming an adequate diet. When macrophages are stimulated with an endotoxin, they produce a factor or factors, termed cachetin, that inhibits the activity of fat-producing (lipogenic) enzymes in cultured adipocytes. This effect may reflect one of the physiological bases for cachexia. In the present study, clones of complementary DNA from genes whose expression is increased during the differentiation of adipocytes were used to study the molecular basis of cachectin''s actions. In the presence of cachectin, the expression of the corresonding genes was reversibly and specifically inhibited. When mature adipocytes were exposed to cachectin, the mRNA of those genes diminished and rapidly approached the levels present before differentiation.