Energy metabolism of blood platelets. I. Isolation and properties of platelet mitochondria.
Energy metabolism of blood platelets. I. Isolation and properties of platelet mitochondria.
复制标题
血小板的能量代谢。
DOI：
10.1016/0003-9861(72)90391-8
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发表时间：
1972
影响因子：
3.9
通讯作者：
M. Fukami
中科院分区：
文献类型：
--
作者：
L. Salganicoff;M. Fukami
本发明描述了一种从猪血小板中分离偶联的线粒体制品的方法，该方法是通过用改良的用于约800 psi压力的French压力池进行破坏来进行的。电子显微照片显示该制剂含有完整的线粒体和α颗粒。该制剂偶联良好; α-酮戊二酸的呼吸控制率为3-7，琥珀酸的呼吸控制率为2-4。氧化最快的底物是α-甘油磷酸，氧化速率高达140 natoms氧/min/mg蛋白质。柠檬酸循环中间产物的氧化速率均不超过20 natoms/min/mg;丙酮酸加苹果酸的呼吸速率仅为4-7 natoms/min/mg。ADP：O的比例与各种底物和呼吸链的反应，以位点特异性抑制剂是相同的，在其他哺乳动物线粒体。连二亚硫酸盐处理的线粒体与有氧线粒体的室温差光谱显示，相对浓度为1：0.54：1.06时，（a+a3）、B和（c1+c）的电子传递链细胞色素的α带分别位于603、560和550 nm处。
A method for the isolation of a coupled mitochondrial preparation from pig platelets by disruption with a French pressure cell modified for use at pressures of approximately 800 psi is described. Electron micrographs showed this preparation to contain intact mitochondria and α-granules. This preparation was well-coupled; respiratory control ratios of 3–7 were obtained with α-ketoglutarate and 2–4 with succinate. The most rapidly oxidized substrate was α-glycerophosphate at rates as high as 140 natoms of oxygen/min/mg of protein. None of the citric acid cycle intermediates were oxidized at rates greater than 20 natoms/min/mg; rates of respiration with pyruvate plus malate were only 4–7 natoms/min/mg. The ADP:O ratios with various substrates and the response of the respiratory chain to site-specific inhibitors were the same as in other mammalian mitochondria. Room temperature difference spectra of dithionite-treated vs aerobic mitochondria showed the α-bands of the cytochromes of the electron transport chain to be at 603, 560, and 550 nm for (a+a3),b, and (c1+c) at relative concentrations of 1 to 0.54 to 1.06, respectively.