EVALUATION OF 4 ANIMAL-MODELS OF INTRARENAL CALCIUM DEPOSITION AND ASSESSMENT OF THE INFLUENCE OF DIETARY SUPPLEMENTATION WITH ESSENTIAL FATTY-ACIDS ON CALCIFICATION
EVALUATION OF 4 ANIMAL-MODELS OF INTRARENAL CALCIUM DEPOSITION AND ASSESSMENT OF THE INFLUENCE OF DIETARY SUPPLEMENTATION WITH ESSENTIAL FATTY-ACIDS ON CALCIFICATION
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DOI：
10.1007/bf00393305
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发表时间：
1995-08-01
影响因子：
--
通讯作者：
SMITH, S
中科院分区：
文献类型：
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作者：
BURGESS, NA;REYNOLDS, TM;SMITH, S
首先，为了确定诱导肾内钙化的令人满意的动物模型，对先前描述的四种肾内钙化动物模型进行了研究，结果表明，向雌性Sprague-Dawley大鼠腹腔内注射10%葡萄糖酸钙最有效。然后，我们研究了这样一个假设，即膳食补充必需脂肪酸可以减少由于腹膜内钙注射而导致的肾内钙化。使用鱼油和月见报春花油的组合，我们证明了肾实质钙化的显著差异，在未补充的动物中为940 +/- 240 μ g Ca/g肾实质干重，在未补充的动物中为320-370 +/- 55-65 μ g Ca/g肾实质干重。补充动物中的薄壁组织（平均值+/- SEM，P < 0.005）。还证明了二十碳五烯酸（EPA）和γ-亚麻酸（GLA）之间存在协同作用：用含有27 mg/ml EPA和67 mg/ml GLA的组合油制剂与食物混合2%的膳食补充剂与含有400 mg/ml EPA或80 mg/ml GLA的油混合4%的食物一样有效。
Firstly, to determine a satisfactory animal model for induction of intrarenal calcification, a study of four previously described animal models of intrarenal calcification was carried out which showed that intraperitoneal injection of 10% calcium gluconate into female Sprague-Dawley rats was most effective. We then investigated the hypothesis that dietary supplementation with essential fatty acids could reduce the intrarenal calcification developing as a result of intraperitoneal calcium injection. Using a combination of fish oil and evening primrose oil, we demonstrated a significant difference in renal parenchymal calcification, which was 940 +/- 240 mu g Ca/g dry weight renal parenchyma in unsupplemented animals and 320-370 +/- 55-65 mu g Ca/g dry weight renal. parenchyma in supplemented animals (means +/- SEM, P < 0.005). It was also demonstrated that there was synergism between eicosapentaenoic acid (EPA) and gamma-linolenic acid (GLA): dietary supplementation with a combined oil preparation containing 27 mg/ml EPA and 67 mg/ml GLA mixed as 2% with food was as effective as oils containing either 400 mg/ml EPA or 80 mg/ml GLA mixed as 4% of food.