Development and characterization of reinforced poly(L-lactide) scaffolds for bone tissue engineering
Development and characterization of reinforced poly(L-lactide) scaffolds for bone tissue engineering
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DOI：
10.1007/s10856-011-4289-4
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发表时间：
2011-05-01
影响因子：
3.7
通讯作者：
Todo, Mitsugu
中科院分区：
文献类型：
--
作者：
Park, Joo-Eon;Todo, Mitsugu
为提高聚乳酸（PLLA）支架的力学性能，开发了固体壳、多孔壳、单梁和双梁增强型PLLA支架。试验结果表明，增强结构的引入有效地提高了混凝土的强度和模量等压缩力学性能。还引入了由增强体、多孔基质和边界层三相组成的线弹性模型，以预测增强支架的压缩模量。对比研究清楚地表明，简单的理论模型可以合理地预测具有三相结构的支架的模量。固体壳和多孔壳增强支架在压缩下的失效机制被发现分别是固体壳的屈曲和在多孔壳中构建孔的支柱的局部屈曲。相反，对于梁加强支架，主要失效机制被理解为梁内的微裂纹和随后由于微裂纹的聚结而形成的主裂纹。生物学研究表明，培养12天后，成骨样细胞MC 3 T3-E1在支架表面粘附和增殖良好。
Novel reinforced poly(L-lactic acid) (PLLA) scaffolds such as solid shell, porous shell, one beam and two beam reinforced scaffolds were developed to improve the mechanical properties of a standard PLLA scaffold. Experimental results clearly indicated that the compressive mechanical properties such as the strength and the modulus are effectively improved by introducing the reinforcement structures. A linear elastic model consisting of three phases, that is, the reinforcement, the porous matrix and the boundary layer was also introduced in order to predict the compressive moduli of the reinforced scaffolds. The comparative study clearly showed that the simple theoretical model can reasonably predict the moduli of the scaffolds with three phase structures. The failure mechanism of the solid shell and the porous shell reinforced scaffolds under compression were found to be buckling of the solid shell and localized buckling of the struts constructing the pores in the porous shell, respectively. For the beam reinforced scaffolds, on the contrary, the primary failure mechanism was understood to be micro-cracking within the beams and the subsequent formation of the main-crack due to the coalescence of the micro-racks. The biological study was exhibited that osteoblast-like cells, MC3T3-E1, were well adhered and proliferated on the surfaces of the scaffolds after 12 days culturing.