Identification and characterization of estrogen receptor-related receptor alpha and gamma in human glioma and astrocytoma cells.
Identification and characterization of estrogen receptor-related receptor alpha and gamma in human glioma and astrocytoma cells.
复制标题
DOI：
10.1016/j.mce.2009.10.001
复制
发表时间：
2010-02-05
影响因子：
4.1
通讯作者：
Wainer, Irving W.
中科院分区：
文献类型：
--
作者：
Gandhari, Mukesh K.;Frazier, Chester R.;Hartenstein, Julia S.;Cloix, Jean-Francois;Bernier, Michel;Wainer, Irving W.
关键词：
本研究的目的是检测雌激素受体相关受体（ers）在人胶质瘤和星形细胞瘤细胞系中的表达和功能。这些雌激素受体阴性的细胞系在不同程度上以细胞环境依赖的方式表达ERRα和ERRγ蛋白，U87MG胶质瘤细胞表达这两种孤儿核受体。在ERR异构体特异性激动剂和拮抗剂存在的情况下进行细胞增殖试验，计算出的EC50和IC50值与之前报道的其他类型癌细胞系的值一致。在这些细胞中也观察到在ERR异构体特异性启动子的控制下诱导荧光素酶的表达。这些结果表明，ERRα和ERRγ在这些肿瘤细胞系中有差异表达，并可能促进受体激动剂依赖性ERR转录活性。
The purpose of this study was to examine expression and function of estrogen receptor-related receptors (ERRs) in human glioma and astrocytoma cell lines. These estrogen receptor-negative cell lines expressed ERRα and ERRγ proteins to varying degree in a cell context dependent manner, with U87MG glioma cells expressing both orphan nuclear receptors. Cell proliferation assays were performed in the presence of ERR isoform-specific agonists and antagonists, and the calculated EC50 and IC50 values were consistent with previous reported values determined in other types of cancer cell lines. Induction of luciferase expression under the control of ERR isoform-specific promoters was also observed in these cells. These results indicate that ERRα and ERRγ are differentially expressed in these tumor cell lines and likely contribute to agonist-dependent ERR transcriptional activity.
登录
查看更多内容
影响因子：
4.8
作者：
Lanvin, Olivia;Bianco, Stephanie;Vanacker, Jean-Marc
通讯作者：
Vanacker, Jean-Marc
影响因子：
11.2
作者：
Hui, AM;Wei, Z;Fine, HA
通讯作者：
Fine, HA
DOI：
10.1016/j.bbrc.2003.12.194
发表时间：
2004-02-20
影响因子：
3.1
作者：
Huppunen, J;Aarnisalo, P
通讯作者：
Aarnisalo, P
影响因子：
1.9
作者：
Mastronardi, L;Farah, JO;Ruggeri, A
通讯作者：
Ruggeri, A
影响因子：
16
作者：
Greschik, H;Wurtz, JM;Renaud, JP
通讯作者：
Renaud, JP