PICKLE chromatin-remodeling factor controls thermosensory hypocotyl growth of Arabidopsis.
PICKLE chromatin-remodeling factor controls thermosensory hypocotyl growth of Arabidopsis.
复制标题
DOI：
10.1111/pce.13049
复制
发表时间：
2017-10
期刊：
影响因子：
--
通讯作者：
P. Zha;Yanjun Jing;Gang Xu;Rongcheng Lin
中科院分区：
文献类型：
--
作者：
P. Zha;Yanjun Jing;Gang Xu;Rongcheng Lin
温度是控制植物分布和塑造植物生长的主要信号。较高的环境温度促进了植物的热形态发生，而没有明显的诱导热胁迫反应。尽管近年来在温度调控植物生长方面取得了很大进展，但对热形态信号转导途径的了解还不是很清楚。我们先前发现，泡菜(PKL)是一种依赖于ATP的染色质重塑因子，它对拟南芥的光形态发生具有负面控制作用。在这里，我们表明PKL的突变导致下胚轴伸长对温度(28摄氏度)的敏感性降低。我们证明了昼夜节律时钟相关1(CCA1)直接与PKL的特定启动子区域结合，并且在CCA1突变体中其表达降低。我们发现，CCA1幼苗对温度调节的生长也不像野生型植物那么敏感。此外，PKL还影响与吲哚-3-醋酸诱导蛋白19(IAA19)和IAA29相关的组蛋白H3Lys 27的三甲基化水平，并调节它们的表达。我们还确定了另外6个转录因子作为PKL的上游调控因子。因此，我们的研究揭示了PKL和CCA1作为两个新的因子来控制植物在高温环境下的生长，并为通过染色质调节光和温度信号的整合提供了新的视角。
Temperature is a major signal that governs plant distribution and shapes plant growth. High ambient temperature promotes plant thermomorphogenesis without significant induction of heat-stress responses. Although much progress of warm temperature-mediated plant growth has been made during recent years, the thermomorphogenic signalling pathway is not well understood. We previously revealed that PICKLE (PKL), an ATP-dependent chromatin remodelling factor, negatively controls photomorphogenesis in Arabidopsis thaliana. Here, we show that mutations in PKL lead to reduced sensitivity in hypocotyl elongation to warm temperature (28 °C). We demonstrate that CIRCADIAN CLOCK-ASSOCIATED 1 (CCA1) directly binds to the specific promoter regions of PKL and its expression is reduced in the cca1 mutants. We find that the cca1 seedlings are also less sensitive to temperature-mediated growth than the wild-type plants. Furthermore, PKL affects the level of trimethylation of histone H3 Lys 27 associated with INDOLE-3-ACETIC ACID INDUCIBLE 19 (IAA19) and IAA29 and regulates their expression. We also identify 6 additional transcription factors as the upstream regulators of PKL. Our study thus reveals PKL and CCA1 as 2 novel factors in controlling plant growth in response to the elevated temperature environment and provides new insight into the integration of light and temperature signals through chromatin regulation.