Androgens promote insulin-like growth factor-I and insulin-like growth factor-I receptor gene expression in the primate ovary
Androgens promote insulin-like growth factor-I and insulin-like growth factor-I receptor gene expression in the primate ovary
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DOI：
10.1093/humrep/14.9.2328
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发表时间：
1999-09-01
影响因子：
6.1
通讯作者：
Bondy, CA
中科院分区：
文献类型：
--
作者：
Vendola, K;Zhou, J;Bondy, CA
最近的研究表明，雄激素会促进灵长类动物卵巢中未成熟卵泡的生长。在本研究中，研究了雄激素对卵巢胰岛素样生长因子I（IGF-I）和IGF-I受体基因表达的影响。研究组包括五个卵泡期、安慰剂治疗的对照组，以及四只睾酮和三只二氢睾酮（DHT）治疗的恒河猴。治疗周期为5天。睾酮和 DHT 治疗均导致颗粒层、鞘膜和间质室中 IGF-I mRNA 浓度显着增加 3-4 倍。同样，两种雄激素均诱导 IGF-I 受体 mRNA 量显着增加，最显着的是在鞘细胞中，而在颗粒层和间质中则不太明显（P < 0.05）。 IGF 系统 mRNA 量的这些变化在生长中的卵泡直至小窦（直径小于或等于 1 毫米）阶段都有记录。较大的卵泡数量太少，无法进行显着比较。相比之下，雄激素治疗并没有明显改变卵巢胰岛素受体 mRNA 的量。这些数据表明IGP-I及其同源受体可能介导雄激素诱导的卵泡和膜间质生长。
It has recently been shown that androgens increase the growth of immature follicles in the primate ovary. In the present study the effect of androgens on ovarian insulinlike growth factor I(IGF-I) and IGF-I receptor gene expression was investigated. The study groups included five follicular phase, placebo-treated controls, and four testosterone- and three dihydrotestosterone (DHT)-treated rhesus monkeys. The treatment period was 5 days. Both testosterone and DHT treatment resulted in significant, 3-4-fold increases in IGF-I mRNA concentration in granulosa, thecal and interstitial compartments. Likewise, both androgens induced significant increases in the amount of IGF-I receptor mRNA, most notably in thecal cells and less markedly in granulosa and interstitium (P < 0.05). These changes in amounts of IGF system mRNA were documented in growing follicles up to the small antral (less than or equal to 1 mm diameter) stage. Larger follicles were too few in number for significant comparisons. By contrast, amounts of ovarian insulin receptor mRNA were not appreciably altered by androgen treatment. These data suggest that IGP-I and its cognate receptor may mediate androgen-induced ovarian follicular and thecal-interstitial growth.