A STUDY OF THE JELLY ENVELOPES SURROUNDING THE EGG OF THE AMPHIBIAN, XENOPUS LAEVIS

A STUDY OF THE JELLY ENVELOPES SURROUNDING THE EGG OF THE AMPHIBIAN, XENOPUS LAEVIS
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两栖动物非洲爪蟾卵周围果冻膜的研究

DOI:
10.2307/1539712
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发表时间:
1968
期刊:
The Biological Bulletin
影响因子:
--
通讯作者:
S. Freeman
S. Freeman
中科院分区:
--
文献类型:
--
作者:
S. Freeman

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1. 用细胞化学和生物化学方法研究了非洲爪蟾卵周围胶浆的沉积、结构和组成。在通过输卵管的过程中,卵子被包裹着三层果冻,从最里面到最外面,分别被命名为J1、J2和J3。特别强调了对各层的分别分析。各组中性多糖组织化学检测均呈阳性。J2和J3染色最强烈。每层多糖中存在的糖用色谱法鉴定。J1、J2和J3都含有焦糖、葡萄糖胺和半乳糖胺。半乳糖存在于J1和J2,也可能存在于J3。J3也可能含有甘露糖或甘露糖胺。组织化学试验表明J1含有硫酸盐。J2和J3中酸性多糖的弱正反应表明仅存在羧基。由于在果冻中没有发现醛酸,因此有人提出羧基是…
1. The deposition, structure, and composition of the jelly surrounding the egg of Xenopus laevis were studied cytochemically and biochemically. During its passage through the oviduct, the egg is invested with three layers of jelly designated J1, J2, and J3, from innermost to outermost. Particular emphasis was placed on analyzing the layers separately.2. All layers gave positive histochemical tests for neutral polysaccharides. J2 and J3 stained the most intensely. The sugars present in the polysaccharides of each layer were identified chromatographically. J1, J2, and J3 all contain fucose, glucosamine, and galactosamine. Galactose is present in J1 and J2 and possibly in J3. J3 may also contain mannose or mannosamine.3. On the basis of the histochemical tests it is suggested that J1 contains sulfate. The weak positive reactions for acid polysaccharides in J2 and J3 indicate the presence of carboxyl groups only. Since no uronic acid was found in the jelly it is proposed that the carboxyl groups are part of a...