EFFECT OF BRASSINOLIDE AND OTHER GROWTH-REGULATORS ON THE GERMINATION AND GROWTH OF POLLEN TUBES OF PRUNUS-AVIUM USING A MULTIPLE HANGING-DROP ASSAY
EFFECT OF BRASSINOLIDE AND OTHER GROWTH-REGULATORS ON THE GERMINATION AND GROWTH OF POLLEN TUBES OF PRUNUS-AVIUM USING A MULTIPLE HANGING-DROP ASSAY
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DOI：
10.1071/pp9850201
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发表时间：
1985-01-01
期刊：
影响因子：
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通讯作者：
ROWAN, KS
中科院分区：
文献类型：
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作者：
HEWITT, FR;HOUGH, T;ROWAN, KS
用生长抑制剂环己亚胺和生长促进剂丝梭霉素对一种新的多悬滴法进行了试验。该试验用于检测鸟假单胞菌花粉管对油菜素内酯的生长反应，油菜素内酯是一种天然存在的植物生长促进剂。将这种反应与IAA、GA和kinetin进行比较。花粉管对1 nM及以上浓度的油菜素内酯和梭霉素有响应，浓度比吲哚-3-乙酸和赤霉素低1个数量级。激动素不刺激花粉管的生长。
A new multiple hanging-drop assay was tested with a growth inhibitor, cycloheximide, and a growth-promoter, fusicoccin. This assay was used to examine the growth-response of pollen-tubes of P. avium to brassinolide, a naturally occurring promoter of plant growth. This response is compared with that of IAA, GA, and kinetin. Pollen tubes responded to brassinolide and fusicoccin at 1 nM and above, a concentration 1 order of magnitude lower than that for indol-3-ylacetic acid and gibberellic acid. Kinetin did not stimulate growth of the pollen tubes.