High-frequency ultrasound scattering from microspheres and single cells
High-frequency ultrasound scattering from microspheres and single cells
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DOI：
10.1121/1.1830668
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发表时间：
2005-02-01
影响因子：
2.4
通讯作者：
Kolios, MC
中科院分区：
文献类型：
--
作者：
Baddour, RE;Sherar, MD;Kolios, MC
使用高频超声（20-100 MHz）评估感兴趣体积中经历结构变化（例如细胞死亡）的生物细胞的比例，需要开发任何任意细胞系综的散射的理论模型。建立这样一个模型的先决条件是知道不同状态下单个细胞的散射。本文提出了一个简单的单细胞高频声散射模型。推导的方法从一个单一的，亚分辨率散射体的后向散射传递函数也被设计出来。使用这种方法，实验测量的后向散射均匀，亚分辨率聚苯乙烯微球和单一的，活的真核细胞，在广泛的，连续的频率范围内获得的弹性散射理论和建议的细胞散射模型进行了比较，分别。在微球的后向散射传递函数中观察到的共振特征被发现准确地对应于理论预测。使用实验获得的传递函数中的主要光谱峰的间距，可以预测微球直径，误差小于4%。在细胞模型和测量的来自细胞的反向散射之间没有看到这种良好的一致性。这种差异的可能原因进行了讨论。(C)2005年美国声学学会。
Assessing the proportion of biological cells in a volume of interest undergoing structural changes, such as cell death, using high-frequency ultrasound (20-100 MHz), requires the development of a theoretical model of scattering by any arbitrary cell ensemble. A prerequisite to building such a model is to know the scattering by a single cell in different states. In this paper, a simple model for the high-frequency acoustic scattering by one cell is proposed. A method for deducing the backscatter transfer function from a single, subresolution scatterer is also devised. Using this method, experimental measurements of backscatter from homogeneous, subresolution polystyrene microspheres and single, viable eukaryotic cells, acquired across a broad, continuous range of frequencies were compared with elastic scattering theory and the proposed cell scattering model, respectively. The resonant features observed in the backscatter transfer function of microspheres were found to correspond accurately to theoretical predictions. Using the spacing of the major spectral peaks in the transfer functions obtained experimentally, it is possible to predict microsphere diameters with less than 4 % error. Such good agreement was not seen between the cell model and the measured backscatter from cells. Possible reasons for this discrepancy are discussed. (C) 2005 Acoustical Society of America.