Enhancement of nasal absorption of insulin using chitosan nanoparticles
Enhancement of nasal absorption of insulin using chitosan nanoparticles
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DOI：
10.1023/a:1018908705446
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发表时间：
1999-10-01
影响因子：
3.7
通讯作者：
Alonso, MJ
中科院分区：
文献类型：
--
作者：
Fernández-Urrusuno, R;Calvo, P;Alonso, MJ
目的.研究壳聚糖纳米粒作为鼻滴注后改善胰岛素全身吸收的系统的潜力。通过壳聚糖与三聚磷酸盐阴离子的离子凝胶化制备了负载胰岛素的壳聚糖纳米粒。它们的大小和zeta电位分别通过光子相关光谱和激光多普勒风速仪进行了表征。通过microBCA蛋白测定来确定胰岛素负载和释放。在清醒家兔模型上，通过监测血糖水平来研究壳聚糖纳米粒促进胰岛素鼻腔吸收的能力。壳聚糖纳米颗粒的尺寸在300-400 nm范围内，具有正表面电荷，并且它们的胰岛素负载可以被调节达到高达55%的值[胰岛素/纳米颗粒（w/w）：55/100]。发现胰岛素缔合润湿高度介导的离子相互作用机制和其释放在体外迅速发生在水槽条件。壳聚糖纳米颗粒比壳聚糖水溶液更大程度地增强胰岛素的鼻吸收。壳聚糖的量和分子量对胰岛素反应没有显著影响。壳聚糖纳米颗粒是通过鼻粘膜运输胰岛素的有效载体。
Purpose. To investigate the potential of chitosan nanoparticles as a system for improving the systemic absorption of insulin following nasal instillation.Methods. insulin-loaded chitosan nanoparticles were prepared by ionotropic gelation of chitosan with tripolyphosphate anions. They were characterized for their size and zeta potential by photon correlation spectroscopy and laser Doppler anemometry, respectively. Insulin loading and release was determined by the microBCA protein assay. The ability of chitosan nanoparticles to enhance the nasal absorption of insulin was investigated in a conscious rabbit model by monitoring the plasma glucose levels.Results. Chitosan nanopartictes had a size in the range of 300-400 nm, a positive surface charge and their insulin loading can be modulated reaching values up to 55% [insulin/nanoparticles (w/w): 55/100]. Insulin association wets found to be highly mediated by an ionic interaction mechanism and its release in vitro occurred rapidly in sink conditions. Chitosan nanoparticles enhanced the nasal absorption of insulin to a greater extent than an aqueous solution of chitosan. The amount and molecular weight of chitosan did not have a significant effect on insulin response.Conclusions. Chitosan nanopartictes are efficient vehicles for the transport of insulin through the nasal mucose.