Tanshinone IIA and Astragaloside IV promote the angiogenesis of mesenchymal stem cell-derived endothelial cell-like cells via upregulation of Cx37, Cx40 and Cx43.
Tanshinone IIA and Astragaloside IV promote the angiogenesis of mesenchymal stem cell-derived endothelial cell-like cells via upregulation of Cx37, Cx40 and Cx43.
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Tanshinone IIA和Astagaloside IV通过上调CX37，CX40和CX43促进间充质干细胞源性内皮细胞样细胞的血管生成。
DOI：
10.3892/etm.2017.5636
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发表时间：
2018-03
影响因子：
2.7
通讯作者：
Wang W
中科院分区：
文献类型：
--
作者：
Li Z;Zhang S;Cao L;Li W;Ye YC;Shi ZX;Wang ZR;Sun LX;Wang JW;Jia LT;Wang W
丹参酮 IIA (Tan IIA) 和黄芪甲苷 IV (AGS-IV) 在中国古代被用作治疗冠心病 (CHD)。然而，介导 Tan IIA 和 AGS-IV 在血管生成中的作用的潜在机制仍然未知。在本研究中，间充质干细胞（MSC）在体外被诱导分化为内皮细胞（EC）样细胞，并评估了 Tan IIA 和/或 AGS-IV 对这些细胞功能（包括细胞增殖和管形成）的影响。与单药组（仅Tan IIA或AGS-IV）相比，联合药物（Tan IIA和AGS-IV）治疗显着增强EC样细胞的增殖和管形成能力。此外，与单药组相比，联合用药组中连接蛋白37（Cx37）、Cx40和Cx43的表达显着增加。此外，在联合药物组中发现了增强的间隙连接细胞间通讯（GJIC），这一点可以通过刮擦加载染料转移测定中染料转移的增加来证明。总之，Tan IIA和AGS-IV可能通过上调Cx37、Cx40和Cx43的表达以及增强GJIC功能来促进EC样细胞的血管生成。本研究结果可为Tan IIA和AGS-IV治疗先心病的临床应用提供实验证据。
Tanshinone IIA (Tan IIA) and Astragaloside IV (AGS-IV) were used as therapeutic treatments for coronary heart diseases (CHDs) in ancient China. However, the underlying mechanisms mediating the effects of Tan IIA and AGS-IV in angiogenesis remain unknown. In the present study, mesenchymal stem cells (MSCs) were induced to differentiate into endothelial cell (EC)-like cells in vitro and the effects of Tan IIA and/or AGS-IV on the functions of these cells, including cell proliferation and tube formation, were assessed. Compared with the single-agent groups (Tan IIA or AGS-IV only), combined-agent (Tan IIA and AGS-IV) treatment significantly enhanced the proliferation and tube formation capacity of EC-like cells. In addition, the expression of connexin 37 (Cx37), Cx40 and Cx43 in the combined-agent group was significantly increased compared with the single-agent groups. Furthermore, enhanced gap junctional intercellular communication (GJIC) was identified in the combined-agent group, as evidenced by increased dye transfer in scrape-loading dye transfer assays. In conclusion, Tan IIA and AGS-IV may promote the angiogenesis of EC-like cells by upregulating the expression of Cx37, Cx40 and Cx43 and enhancing GJIC function. The results of the present study may provide experimental evidence for the clinical application of Tan IIA and AGS-IV as a treatment for CHDs.
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影响因子：
5.7
作者：
Carlsson, Axel C.;Li, Xinjun;Sundquist, Kristina
通讯作者：
Sundquist, Kristina
影响因子：
9.3
作者：
Gaertner, Christiane;Ziegelhoeffer, Barbara;Dhein, Stefan
通讯作者：
Dhein, Stefan
DOI：
10.1016/s0140-6736(15)00343-8
发表时间：
2015-10-10
期刊：
Lancet (London, England)
影响因子：
--
作者：
Jiang L;Krumholz HM;Li X;Li J;Hu S
通讯作者：
Hu S
影响因子：
4.2
作者：
Gaziano, Thomas A.;Bitton, Asaf;Anand, Shuchi;Abrahams-Gessel, Shafika;Murphy, Adrianna
通讯作者：
Murphy, Adrianna
影响因子：
--
作者：
Alonso F;Domingos-Pereira S;Le Gal L;Derré L;Meda P;Jichlinski P;Nardelli-Haefliger D;Haefliger JA
通讯作者：
Haefliger JA