Differential mucin gene expression in human pancreatic and colon cancer cells.
Differential mucin gene expression in human pancreatic and colon cancer cells.
复制标题
人胰腺癌细胞和结肠癌细胞中的差异粘蛋白基因表达。
DOI：
10.1042/bj2760599
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Kim,YS
中科院分区：
文献类型：
--
作者：
Yonezawa,S;Byrd,JC;Dahiya,R;Ho,JJ;Gum,JR;Griffiths,B;Swallow,DM;Kim,YS
本研究的目的是确定由四种不同的胰腺癌细胞系合成和分泌的粘蛋白的数量和性质。发现良好至中度分化的SW 1990和CAPAN-2人胰腺癌细胞比分化较低的MIA PaCa-2和PANC-1细胞产生更多的高Mr糖蛋白（HMG）。大多数标记的HMG在24 h内分泌。化学和酶降解、离子交换层析和密度梯度离心的结果表明，SW 1990和CAPAN-2细胞中的HMG具有粘蛋白的预期性质，而MIA PaCa-2和PANC-1细胞中的大部分HMG可能不是粘蛋白，而是蛋白多糖。这些结果与免疫印迹和北方印迹一致，显示在SW 1990和CAPAN-2细胞中存在脱粘蛋白和脱粘蛋白mRNA，但在MIA PaCa-2和PANC-1细胞中不存在。Western印迹和北方印迹也显示SW 1990和CAPAN-2细胞与乳腺癌细胞一样，具有由MUC 1基因编码的乳腺型脱粘蛋白和mRNA，但缺乏由MUC 2基因编码的肠型脱粘蛋白和mRNA。相比之下，在培养物中测试的结肠癌细胞系表达由MUC 2编码但不由MUC 1编码的脱粘蛋白和mRNA。
The purpose of this study was to determine the quantity and nature of the mucins synthesized and secreted by four different pancreatic cancer cell lines. Well- to moderately-differentiated SW1990 and CAPAN-2 human pancreatic cancer cells were found to produce more high-Mr glycoprotein (HMG) than less-differentiated MIA PaCa-2 and PANC-1 cells. Most of the labelled HMG was secreted within 24 h. The results of chemical and enzymic degradation, ion-exchange chromatography and density-gradient centrifugation indicated that the HMG in SW1990 and CAPAN-2 cells has the properties expected for mucins, whereas much of the HMG in MIA PaCa-2 and PANC-1 cells may not be mucin, but proteoglycan. These results are consistent with immunoblots and Northern blots showing the presence of apomucin and apomucin mRNA in SW1990 and CAPAN-2 cells, but not in MIA PaCa-2 and PANC-1 cells. The Western blots and Northern blots also show that SW1990 and CAPAN-2 cells, like breast cancer cells, have the mammary-type apomucin and mRNA coded by the MUC1 gene, but lack the intestinal type apomucin and mRNA coded by the MUC2 gene. In contrast, the colon cancer cell lines tested in culture express apomucin and mRNA coded by MUC2 but not by MUC1.