Methyloligella halotolerans gen. nov., sp nov and Methyloligella solikamskensis sp nov., two non-pigmented halotolerant obligately methylotrophic bacteria isolated from the Ural saline environments
Methyloligella halotolerans gen. nov., sp nov and Methyloligella solikamskensis sp nov., two non-pigmented halotolerant obligately methylotrophic bacteria isolated from the Ural saline environments
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DOI：
10.1016/j.syapm.2012.12.001
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发表时间：
2013-05-01
影响因子：
3.4
通讯作者：
Trotsenko, Yuri A.
中科院分区：
文献类型：
--
作者：
Doronina, Nina V.;Poroshina, Maria N.;Trotsenko, Yuri A.
本文报道了两株新分离的耐盐专性甲基营养菌（C2（T）和SK 12（T）），它们具有C-1丝氨酸同化途径。分离物是严格好氧的，革兰氏阴性，无孢子的，不运动的棒，形成棒状，通过二分裂繁殖。嗜中性和嗜酸性，积累细胞内相容的溶质四氢嘧啶和聚-β-羟基丁酸酯。新菌株能够在0至16%NaCl（w/v）下生长，最佳在3- 5%NaCl下生长。主要的细胞脂肪酸是C-18：1 omega 7 c和C-19：0 cyc，主要的醌是Q-10。主要的磷脂是磷脂酰胆碱、磷脂酰甘油、磷脂酰二甲基乙醇胺和磷脂酰乙醇胺。通过谷氨酸脱氢酶同化NH 4+。以及通过谷氨酸循环（谷氨酰胺合成酶和谷氨酸合成酶）。菌株C2（T）和SK 12（T）的DNA G + C含量分别为60.9和60.5mol%（Tm）。这两个新分离株的16 S rRNA基因序列相似性为99%，但与Alphaproteobacteria的其他成员的相似性低于94%，因此表明它们可以被分配到一个新的属Methyloligella。菌株C2（T）和SK 12（T）之间相当低水平的DNA-DNA相关性（53%）表明它们代表新属的两个独立种，新种Methyloligella solikamskensis sp. nov.。耐盐甲基寡孢菌的模式菌株为C2（T）（=VKM B-2706（T）= CCUG 61687（T）= DSM 25045（T）），索利卡姆斯基甲基寡孢菌的模式菌株为SK 12（T）（=VKM B-2707（T）= CCUG 61697（T）= DSM 25212（T））。(C)2012 Elsevier GmbH. All rights reserved.
Two newly isolated halotolerant obligately methylotrophic bacteria (strains C2(T) and SK12(T)) with the serine pathway of C-1 assimilation are described. The isolates are strictly aerobic, Gram negative, asporogenous, non-motile rods, forming rosettes, multiplying by binary fission. Mesophilic and neutrophilic, accumulate intracellularly compatible solute ectoine and poly-beta-hydroxybutyrate. The novel strains are able to grow at 0 up to 16% NaCl (w/v), optimally at 3-5% NaCl. The major cellular fatty acids are C-18:1 omega 7c and C-19:0cyc and the prevailing quinone is Q-10. The predominant phospholipids are phosphatidylcholine, phosphatidylglycerol, phosphatidyldimethylethanolamine and phosphatidylethanolamine. Assimilate NH4+ by glutamate dehydrogenase. and via the glutamate cycle (glutamine synthetase and glutamate synthase). The DNA G + C contents of strains C2(T) and SK12(T) are 60.9 and 60.5 mol% (Tm), respectively. 16S rRNA gene sequence similarity between the two new isolates are 99% but below 94% with other members of the Alphaproteobacteria thus indicating that they can be assigned to a novel genus Methyloligella. Rather low level of DNA-DNA relatedness (53%) between the strains C2(T) and SK12(T) indicates that they represent two separate species of the new genus, for which the names Methyloligella halotolerans gen. nov., sp. nov. and Methyloligella solikamskensis sp. nov. are proposed. The type strain of Methyloligella halotolerans is C2(T) (=VKM B-2706(T) = CCUG 61687(T) = DSM 25045(T)) and the type strain of Methyloligella solikamskensis is SK12(T) (=VKM B-2707(T) = CCUG 61697(T) = DSM 25212(T)). (C) 2012 Elsevier GmbH. All rights reserved.