Interactions between cro repressor and the model specific binding site
Interactions between cro repressor and the model specific binding site
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Cro 阻遏物与模型特异性结合位点之间的相互作用
DOI：
10.1016/0014-5793(84)80759-0
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发表时间：
1984
期刊：
影响因子：
3.5
通讯作者：
K. Skryabin
中科院分区：
文献类型：
--
作者：
M. P. Kirpichnikov;A. Kurochkin;B. Chernov;K. Skryabin
用质子核磁共振波谱研究了λ噬菌体cro阻遏物与OR 3合成的一半（特异性结合位点中最保守的一半）的结合。发现蛋白质的α螺旋片段（27-36）参与与模型结合位点的特异性相互作用。cro阻遏物的三维结构在复合物形成时不会发生明显变化。可以排除插入作为可能的相互作用手段。
Binding of λ phage cro repressor to the synthetic half of OR3, the most conservative half of the specific binding sites, was investigated by proton nuclear magnetic resonance spectroscopy. It was found that the α‐helical segment (27–36) of the protein was involved in specific interactions with the model binding site. The 3‐dimensional structure of cro repressor does not change noticeably upon complex formation. Intercalation can be excluded as a possible means of interaction.